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Floral Dip: Agrobacterium-Mediated Germ Line Transformation

 Last Update: 2020-11-12
 Source: Internet
 Author: User

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essential genes

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Many researchers use the flowering plant
Arabidopsis thaliana
to study gene function and basic plant biology. This easy-to-grow, small plant is ideal for genetic studies as it has a relatively simple genome compared to crop plants and its genetic material has been recently sequenced. Another very useful feature of
Arabidopsis
is that it is extremely simple to transform genetically. The ability to insert genes of interest stably into a given plant is essential to understand and verify gene function. Transformation is also a means of introducing specific traits that are difficult or impossible to introduce by conventional breeding techniques. This chapter provides detailed explanations on the floral dip protocol, a simple method to transform
Arabidopsis
by inoculating immature flowers with
Agrobacterium tumefaciens
.

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