Fierce! Back to back two pieces of Science in the same research group: revealing the relationship between sleep and synaptic rhythm

October 14, 2019 / BIOON / -- Ludwig Maximilians university? T, LMU's time biologist pointed out in the two articles of "Science" that the sleep wake cycle is crucial to the protein and phosphorylation kinetics regulating the activity of synapses in synapses The biological clock controls almost all the physiological processes of human body and predicts the environmental changes of daily cycle such as day and night The molecular mechanism of how circadian rhythm and sleep affect the level of brain cells remains unclear Professor Maria Robles, group leader of LMU medical psychology research team, recently published two articles in the journal Science, revealed how sleep and wakefulness cycles (rather than biological clocks) drive protein abundance in the brain and the cycle of synaptic protein phosphorylation to organize synaptic activity "Our research shows that sleep wake cycle plays a central role in time regulation of many aspects of synaptic function," said Maria Robles Source: Maria Robles, a time biologist at max Iglesias LMU, and her team used quantitative proteomics based on mass spectrometry to map the daily dynamics of protein and phosphorylation in isolated synapses in the mouse forebrain In these studies, the team investigated how synaptoproteins and phosphoproteomes form dynamically during the day, and the effects of sleep deprivation on them In one study, the team found that a quarter of the 8000 key synaptic proteins phosphorylated on a normal day had two major peaks: one when the mice woke up and the other before they went to bed "This suggests that synaptic phosphorylation plays a key role in regulating synaptic function, especially during the sleep wake sleep transition," said Maria Robles "The pattern of phosphorylation of this feature seems to reflect the accumulation and dissipation of sleep and waking pressure, as sleep deprivation almost completely eliminates the synaptic phosphorylation rhythm "Our research shows that key synaptic processes are regulated by phosphorylation when it comes to sleep and wake-up stress," said Maria Robles "In the second study, published in the same issue of science, the same team worked with a team at the University of Zurich (Steve Brown) to show that synaptic protein abundance is also controlled rhythmically by the sleep wake cycle In particular, they showed that synaptic activity triggers the cycle of messenger proteins, which gather at the synapse in a rhythmic fashion throughout the day Although the production of proteins depends entirely on the awake sleep cycle, the travel and accumulation of messenger molecules in synapses are mainly in response to the mechanism of circadian rhythm Reference materials: F bruning El Al., "sleep wake cycles drive daily dynamics of synthetic photography," Science (2019) Doi: 10.1126/science.aav3617 S.B noya El Al., "the forest synthetic transcription is organized by clocks but its protocol is driven by sleep," Science (2019) Doi: 10.1126/science.aav2642 C cirelli el al“ Linking the need to sleep with synaptic function ，" Science (2019) DOI： 10.1126/science.aay5304