Experiment 78 Light cycle induction of Aoping 6746, Japan

Experiment 78 Light Cycle InductionPrincipleJapan Aoping 6746 (Lemna Paucicostata 6746) belongs to Lemnaceae, a short-term sun plant, floating on the surface of the water, with strong reproductive capacity and short growth cycles when conditions are right.
culture
can blossom in 17 days. Because the plant is small, it is beneficial to cultivate under artificial control conditions, which is a very good experimental material.Instrument drugsautoconsulation
pot
ultra-clean work table
or inoculation box hychometer anatomical mirror stration temperature
culture chamber timing switch black cloth Hutner
medium
(see Schedule 16)triangle
sheat bottle
long tweezers1. A large number of elements and trace elemental storage fluids in Hutner mediums are prepared at 100x concentration and stored in the refrigerator.10 ml each of the large number of elements and trace elements were added to about 900 ml of distilled water, 10g of sucrose was added, and the content was fixed to 1000ml. Then remove 800ml plus distilled water to 1000ml, i.e. get 8/10 concentration Hutner medium, adjust pH to 5.6-6.0, divided into triangular bottles, 40ml per bottle. Plug in the cotton plug and wrap in d'ostish with psoria for 20 minutes at high pressure (pressure 1kg/cm2).2． On the ultra-clean workbench (or inoculation box) according to the sterile operating requirements, with long tweezers to access a 3-leaf form of Qingping 6746.3． After vaccination, it is placed under long sun conditions (8 hours dark, 16 hours of light per day), with a light intensity of 2000lx, a culture chamber temperature of 26-27 degrees C, and a culture of 7-8 days.4． Qingping 6746 will be cultivated under long sun conditions for 7-8 days, transferred to a fresh medium, and transferred every 3-4 days there year. Short lighting for different days is performed as shown in the table below, with 5 repetitions for each process.5． At the end of each treatment, it is still cultured back in long lighting conditions, paying attention to observe the effects of different treatments on flowering. Generally from the first day of treatment, to the 9th day after a suitable number of days of short light treatment of the leaf-like edge crack, you can see a needle-sized small yellow flower (Figure 27), daily record the number of flowering leaf-like groups, until the number of flowers decreased.
6． If time permits, you can observe the flower sequence. Qingping 6746 usually each leaf-shaped group has a flower sequence, a few have 2 or 3, can be carefully removed under the anatomical mirror flower sequence, flowers for a single flower, male and female, each flower sequence has 2 male flowers, 1 female flower, has a round spoon-shaped diaphragm. Male flowers are made up of 1 male and are spent in room 4 (Figure 28).