Experiment 66 Effect of ethylene on the shedding of cotton leaves

Experiment 66 Effect of ethylene on cotton leaf sheddingPrincipleEthylene enhances the activity of gelase and cellulose enzyme, accelerates glial and cellulose water contention, and weakens the binding force between cells, resulting in off-layer production, causing organ shedding.ethylene (2-chloroacetylene) in plant cell fluid pH conditions (generally >pH4), slowly liberated ethylene, with the same physiological effects as ethylene.。Instrument medicinescissors tweezersculture
dishes small
beech skimmed cotton ethylene operation step 1. Take cotton seedling plant one, cut off when the body left a leaf handle, and the leaf handle cut short, as shown in Figure 24 (can also use leaf-to-raw plants as a material), on the left and right sides of the leaf shank cut, wrapped with a little skimmed cotton, Drop 1 drop of ethylene solution on the right inlet cotton, 1 drop of distilled water on the left inlet, and the concentration of ethylene is 10, 5, 1, 0.5, 0.05 ppm. Repeat 2 times for each treatment, insert all the treated material in the wet sand of the petri dish, and after 24 hours, touch the leaf handle with a tweezer to see if it falls off. Later every day morning and evening with tweezers to check the fall off, write down the time required for each leaf handle to fall off, compare the results.
。 2. Take the leaves to the raw plant branches, leave three sections, the rest cut off, and three sections of the leaves, cut off the leaf body, leaving the leaf handle. In the middle of a pair of leaf shank cut on a little skimmed cotton, in the right inlet drop on ethylene, the left inlet drop on distilled water, ethylene concentration of 10, 5 ppm, the material inserted in a small berries steamed water, after daily with tweezers to check the three pairs of leaf shank shedding, comparing the results.3. The same test was carried out with different concentrations (10, 5, 1, 0.5, 0.05 ppm) of shedding acid to observe the shedding effect on cotton leaves. The shedding acid is dissolved with a small amount of sodium bicarbonate and diluted with distilled water.。