Experiment 31 Plant pigment and its properties

Experiment 31 Plant Pigment and Its PropertiesPrinciple Plant pigments include fat-soluble chlorophyte pigment and water-soluble cytochrome, the former of which is present in chlorophytes, which are related to photomassing, such as chlorophytes, and the latter in liquid bubbles, especially in the color of flowers, such as anthocyanins. Understanding their nature helps to understand their physiological function.。Instrument drugshydrometer
balance research sub-fluid
flowerpipe
measuring tube sorption ball
test tube luminium bicarbonateacetone ethyl ethermethanol hydrochloric acidcopper acetateoperation step 1. The extraction of chlorophyl pigmenttake spinach (or other plants) leaves 2g, placed in the research, add quartz sand and calcium carbonate a little, acetone about 5 ml, grind into a uniform slurry, and then add acetone 15 ml, then dark green extract, with funnel
filtering
of which is pigment extract.2. Fluorescence of chlorosteroidsTake the above pigment acetone extract a little bit in the test tube, with reflected light and transmission light, to observe the color of the extract is different, reflected light observed solution color, that is, chlorosterone produced fluorescent color.3. The destructive effect of light on chlorosteroidstake a little of the above pigment acetone extract, divided into 2 test tubes, 1 test tube in the dark (or wrapped in black paper), 1 test tube placed in bright light (sunlight), after 2-3 hours, to observe the two test tubes in the color of the solution is different?4. Alternative role of copper in chloroplast molecules take the above pigment acetone extract a little in the test tube, 1 drop of 1 drop of thick hydrochloric acid, until the solution appears brownish-green, at this time chloroplast molecules have been destroyed, forming a magnesium chloroplast. Then add 1 small piece of copper acetate crystal,
to
the solution, and produce bright green. This indicates that copper has replaced the original magnesium position in chloroplast molecules. 5. Separation of yellow and green take the above pigment acetone extract 10 ml, add to a liquid funnel containing 20 ml ether, shake the fractional funnel, and along the edge of the funnel to add 30 ml distilled water, gently shake the fractional funnel, sit still for a moment, the solution is divided into two layers. The pigment has been transferred to the upper ether, discarding the lower acetone and water, and then rinsing the ether solution with distilled water 1-2 times. Then add 5 ml 30% KOH methanol solution to the pigment ether solution, shake the fractional funnel with force, set aside for about 10 minutes, then add distilled water about 10 ml, shake and then sit apart, then get the yellow layer and the green layer, respectively, stored in the test tube. 6. Observe the absorption of pigment solutions
spectral
. (1) adjust the spectrometer to observe the spectrum of electrical light. (2) observe the pigment acetone extract and dilute the solution by 1x with acetone. (3) observe the yellow ether solution and dilute the solution by 1x with ether. (4) to observe the saponified yelorin methanol solution, with methanol to dilute the solution 1 times compared. (5) observe the light-damaged pigment acetone solution and compare it with (2). (6) observe the pigment solution that has been replaced by magnesium by copper. 。