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Eucalyptus

 Last Update: 2021-01-19
 Source: Internet
 Author: User

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medicines derived plants

polymerase chain reaction steps

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Using
Eucalyptus camaldulensis
as a model system, we describe here a basic
Agrobacterium
-mediated genetic transformation protocol through organogenesis for the production of transgenic plants. Hypocotyl segments or cotyledon pieces from in vitro seedlings are used as starting materials. The explants are inoculated and cocultivated with a disarmed, binary strain of
A. tumefaciens
CIB542 harboring a mini Ti plasmid, pBI121. A modified Gamborg’s B5 medium is used as the basal culture medium throughout stages of co-cultivation, callus induction and shoot regeneration. The incorporation of neomycin phosphotransferase II (
npt
II) and β-glucuronidase (
gus
) genes into the plant nuclear genome are primarily verified by histochemical analysis and polymerase chain reaction (
PCR
). Modifications of this protocol to use in mature tissues derived from elite trees and other
Eucalyptus
species are also described.

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Eucalyptus sugar

03-07

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