 Home > Biochemistry News > Plant Extracts News > Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics

Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics

 Last Update: 2021-01-19
 Source: Internet
 Author: User

Tags

1 4 fitting

dna

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

The plastids of higher plants have their own ∼120–160-kb genome that is present in 1,000–10,000 copies per cell. Engineering of the plastid genome (pt
DNA
) is based on homologous recombination between the plastid genome and cloned ptDNA sequences in the vector. A uniform population of engineered ptDNA is obtained by selection for marker genes encoded in the vectors. Manipulations of ptDNA include (1) insertion of transgenes in intergenic regions; (2) posttransformation excision of marker genes to obtain marker-free plants; (3) gene knockouts and gene knockdowns, and (4) cotransformation with multiple plasmids to introduce nonselected genes without physical linkage to marker genes. Most experiments on plastome engineering have been carried out in the allotetraploid
Nicotiana tabacum
. We report here for the first time plastid transformation in
Nicotiana sylvestris
, a diploid ornamental species. We demonstrate that the protocols and vectors developed for plastid transformation in
N. tabacum
are directly applicable to
N. sylvestris
with the advantage that the
N. sylvestris
transplastomic lines are suitable for mutant screens.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Ginsenoside-Rg5

CAS No.: 186763-78-0

Industrial grade

$1/KG EXW

3-O-Ethyl-L-ascorbic acid /86404-04-8

CAS No.: 86404-04-8

pharmaceutical Grade

$10/KG FOB

TETRAHYDROPIPERINE / 23434-88-0

CAS No.: 23434-88-0

pharmaceutical Grade

$10/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.