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bacteria express the full-length SARS-CoV-2 nsp12 (residual S1-Q932) with nsp7 (residual S1-Q83) and nsp8 (residual A1-Q198) incubated together, and then pureized compound formation.
in the presence of this compound to prepare a low-temperature electroscope mesh, the initial screening found that the mesh has good dispersion and excellent particle density.
after collecting and processing 7,994 microscopic films, the researchers achieved a three-dimensional reconstruction of the nsp12 monomer and nsp8 monomers, respectively, at a resolution of 2.9 E, as previously observed in SARS-CoV.
except nsp1