-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Immunoassays were first developed over 30 yr ago. The radioimmunoassay for insulin described by Yalow and Berson (
1
) heralded a new era in the use of antibody reagents for the quantification of proteins and peptides. The Nobel Prize-winning research revolutionized analysis by virtue of much improved specificity and sensitivity coupled with ease of application to large numbers of samples. Subsequently, a number of further innovations have increased the power and utility of immunoassay. Of particular importance was the methodology for generating monoclonal antibodies described by Kohler and Milstein (
2
), theoretically allowing the production of unlimited amounts of identical antibodies (as opposed to the mixed populations making up polyclonal preparations). Second, the use of enzyme-linked immunosorbent assays (ELISAs) has obviated the need for handling radioisotopes (
3
) and consequently widened the utilization of immunoassays from clinical research to virtually all areas of biological analysis (
4
). More recently, the production of recombinant antibody fragments (
5
), using a variety of expression systems, offers the next leap forward. Such technical innovations will offer a faster and more reliable means to synthesize reagents of the desired affinity and specificity, taking immunotechnology on into the next century (
6
).
