Elimination strategy of dry cargo liquid matrix effect

Matrix effects can have a serious impact on the limit of quantification (LOQ), limit of detection (LOD), linearity, accuracy and precision of the method

01

Choose the appropriate sample preparation method

The degree of extraction and purification of the sample solution before it is put on the machine determines the difference in the matrix composition and residual quantity in the sample solution, resulting in different degrees of matrix effect

02

Choose the right chromatographic separation conditions

Because the matrix effect in LC-MS/MS analysis is caused by the co-eluting components of the analyte affecting the ionization efficiency of the electrospray interface

03

Optimizing mass spectrometry analysis conditions

Experiments have shown that the matrix effect of most compounds has a significant impact on ESI, but has little or no impact on APCI and APPI

04

Use appropriate internal standards

Isotope internal standard is the first choice to eliminate matrix effect.

05

Calibration with matrix standard solution

Matrix standard solution, that is, after the blank sample is pre-processed, a certain amount of analyte standard is added to calibrate the test results
.
Calibration with matrix standard solutions is the most commonly used method to compensate for matrix effects
.
It is widely used in the research of liquid chromatography-tandem mass spectrometry at home and abroad
.

In summary, in the development of specific liquid chromatography-tandem mass spectrometry methods, the pretreatment methods must first be optimized according to the nature of the target compound, matrix type, detection limit requirements, etc.
, and the purification of various pretreatment methods should be compared.
The effect, taking into account the matrix effect and recovery rate, eliminate as many endogenous substances as possible that can cause the matrix effect;
secondly, select the appropriate ion source and optimize the chromatographic conditions to obtain the best separation effect and avoid co-elution Impact
.
Selecting the appropriate internal standard and using the matrix standard solution to calibrate is to compensate for the matrix effect generated to offset the matrix effect.
It is a compensation measure when the matrix effect cannot be eliminated by the pre-processing and instrument conditions to ensure the test results.
Accuracy
.