Edible bacteria species are preserved

excellent
strains
, such as poor storage, will occur life force weakened, clutter pollution and other conditions. Therefore, how to maintain the excellent characteristics of bacteria for a long time, not aging and sterilization, is very important work.there are many ways to preservestrains, the principle is to use
dyring
, low temperature, freezing or reducing oxygen supply and other methods to stop its reproduction, reduce its metabolic intensity, so that it is in a sleeping dead state. The following are commonly used:(i) slope low temperature preservation methodstrains in the appropriate slope
culture
base culture mature, moved into the low temperature of 4 degrees C to 6 degrees C preservation, after every 2 to 3 months. This method is suitable for general edible bacteria. If there are no conditions, no refrigerator, you can store the oblique bacteria species replaced with sterile rubber plugs, and with candle seals placed in a closed wide-mouth bottle, suspended into the bottom of the well to preserve.It should be noted that the tolerance of mushrooms to low temperature is poor, its mycelium is easy to die below 5 degrees C, so the storage temperature of mushroom species needs to be raised to 10 degrees C-12 degrees C, or infusion of 3 to 4 ml of antifreeze on the mushroom moss - 10% glycester, so that it can be preserved.(ii) Liquid paraffin storageoutside
test tube


filling base
mycelium plump, in the sterile box, according to the sterile operation method of the sterile liquid paraffin, with sterile
sorptors
injected into it, the injection volume is 1 cm higher than the slope. Then outsource the cotton plug on plastic paper, upright into the refrigerator and indoor drying place, generally can be stored for about 1 year.liquid paraffin preservation principle, can make the bacteria isolated from the outside air, so that the metabolic activity of the bacteria greatly decreased, can prevent aging of the bacteria.(iii) freeze-drying preservation method This method uses vacuum, drying, low temperature and other means, so that the metabolic activity of the bacteria in a relatively static state, at low temperature rapid freezing, and at low temperature vacuum drying, and so that the structure and composition of the bacteria cells to maintain the original state and preserve the species.there are sand and soil preservation method, liquid nitrogen ultra-low temperature preservation method,
filtration paper
piece preservation method, mycelium physiological salt water preservation method, wheat grain preservation method, dry spore vacuum preservation method. It's not all about it. Depending on your job needs and specific conditions. While saving, while also separating, strong, do a good job of breeding, continue the bacteria of the worry benign energy.。