DNA Labeling by Isolated Wheat Chloroplasts
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Last Update: 2021-01-02
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Source: Internet
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Author: User
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In wheat, growth is from a basal meristem: The cells of the young shoot form a developmental array from the tip (oldest) to the base (youngest) of the leaf. In cells from a specific part of the leaf (and so of a certain age), chloroplast
DNA
is found to be replicating. In seedlings up to 10 d old, chloroplast DNA replication is found in the region from 1 to 4 cm from the leaf base (
1
) If chloroplasts are isolated from this region of leaf and incubated in the presence of a radioactively labeled DNA precursor, radioactively labeled DNA can be produced in vitro.
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