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The commonly used detectors for second-hand liquid chromatographs are UV detectors and diode array detectors.
Most liquid chromatographs are equipped with this detector, and most drugs respond in this detector
.
Understand the difference between liquid phase UV detectors and diode array detectors from the aspects of function, working principle and applicable fields
.
1.
UV absorption detectors with different functions are referred to as UV detectors.
They are detectors designed based on the principle that solute molecules absorb UV light
.
Diode array detectors are photodiode array tube detectors, also known as photodiode array detectors or photodiode matrix detectors
.
2.
Different working principles The working principle of the UV absorption detector is the Lambert-Beer law, that is, when a beam of monochromatic light passes through the flow cell, if the mobile phase does not absorb light, the absorbance A and the concentration of the light-absorbing component C and The light path length L of the flow cell is proportional
.
Add Eltech scintillation crystal to the front of the diode array to convert the radiation into visible light, and then use the PCB1024 AD and USB module to convert it into electronic signals, which can also be used in the detection of radiation
.
3.
The use coverage of different UV detectors reaches 75% of HPLC detectors, and has been widely used in various fields, especially in the fields of medicine, environmental protection, life science, food science, agricultural science, food science, medical and health, etc.
, the application is more extensive
.
A series of photodiodes are closely arranged on crystalline silicon, and each diode is equivalent to the exit slit of a monochromator.
The more diodes, the higher the resolution.
Generally, one diode corresponds to receiving monochromatic light with a nanometer spectral bandwidth on the spectrum
.
Most liquid chromatographs are equipped with this detector, and most drugs respond in this detector
.
Understand the difference between liquid phase UV detectors and diode array detectors from the aspects of function, working principle and applicable fields
.
1.
UV absorption detectors with different functions are referred to as UV detectors.
They are detectors designed based on the principle that solute molecules absorb UV light
.
Diode array detectors are photodiode array tube detectors, also known as photodiode array detectors or photodiode matrix detectors
.
2.
Different working principles The working principle of the UV absorption detector is the Lambert-Beer law, that is, when a beam of monochromatic light passes through the flow cell, if the mobile phase does not absorb light, the absorbance A and the concentration of the light-absorbing component C and The light path length L of the flow cell is proportional
.
Add Eltech scintillation crystal to the front of the diode array to convert the radiation into visible light, and then use the PCB1024 AD and USB module to convert it into electronic signals, which can also be used in the detection of radiation
.
3.
The use coverage of different UV detectors reaches 75% of HPLC detectors, and has been widely used in various fields, especially in the fields of medicine, environmental protection, life science, food science, agricultural science, food science, medical and health, etc.
, the application is more extensive
.
A series of photodiodes are closely arranged on crystalline silicon, and each diode is equivalent to the exit slit of a monochromator.
The more diodes, the higher the resolution.
Generally, one diode corresponds to receiving monochromatic light with a nanometer spectral bandwidth on the spectrum
.
