Diabetes res Clin practice: Peking University

Spectral data of all samples In order to explore whether urinary peptide as a biomarker can distinguish type 2 diabetes mellitus patients (T2DM) from healthy controls, Professor Zhang man and his team from the ninth Clinical School of Peking University conducted a study (identification of urinary biomarkers for type 2 diabetes using bed based protocol Approach), the study found that histidine trimer nucleotide binding protein 1, bifunctional aminoacyl tRNA synthetase and aggregator precursor protein can be used as potential biomarkers to distinguish type 2 diabetes from healthy controls The results of the study were published in the August 2013 "diabetes research and clinical practice", with an impact factor of 2.741 In this study, random urine samples were obtained from 28 T2DM patients and 29 healthy individuals After purification with weak cation exchange beads (mb-wcx), they were analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) Then the mass spectrum of the peptide was analyzed by clinprotools 2.1 bioinformatics software Subsequently, the amino acid sequence of the differentially expressed polypeptide was determined by HPLC-MS, and the corresponding protein name was found by sequence search The results showed that three kinds of differentially expressed polypeptides and their mass to charge ratio (M / z) were found Compared with the healthy controls, the peak areas of three differentially expressed peptides in T2DM patients were reduced, and the M / Z were 1056.1 (M / z), 1963.5 (M / z) and 2123.5 (M / z), respectively These peptides were further identified as fragments of histidine trimer nucleotide binding protein 1 (HINT1), bifunctional aminoacyl tRNA synthetase (EPRs) and aggregator precursor protein (clu) It was found that histidine trimer nucleotide binding protein 1, bifunctional aminoacyl tRNA synthetase and aggregator precursor protein could be used as potential biomarkers to distinguish type 2 diabetes from healthy controls.