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"In a sense, insect researchers get rid of the trouble of injecting eggs," said senior study author Takaaki Daimon of Kyoto Universi.
Current insect gene-editing methods often require microinjection of material into early embryos, which severely limits its application in many speci.
To overcome these limitations, Daimon and his collaborators injected Cas9 ribonucleoproteins (RNPs) into the body cavity of adult female cockroaches to introduce genetic mutations in developing egg cel.
The successful application of DIPA-CRISPR on two evolutionarily distant species shows its potential for broad applicati.
Despite these limitations, DIPA-CRISPR is accessible, highly practical, and can be easily implemented in the laboratory, extending the application of gene editing to a wide range of model and non-model insect speci.
"By improving the DIPA-CRISPR method, making it more efficient and versatile, we may be able to enable genome editing in nearly all of the more than 5 million insect species, opening up a future where we can take full advantage of the amazing biological capabilities of insects," Daimon sa.
Yu Shirai, Maria-Dolors Piulachs, Xavier Belles, Takaaki Daim.
