Dev Cell. Miao Guangxia and others have revealed for the first time the molecular mechanisms of new connections between cells in the body.

cell migration is a common process in the development of organisms. !----during development, cells can migrate in a single, small cluster, or in large numbers.maintain dynamic connections between cells during migration.After the migration is complete, the migrated cells must establish a stable new connection with the new cells at the end of their movement.a large number of studies focused on the mechanism of cell migration, relatively, after the cell completes migration, how to establish a new connection with the surrounding cells mechanism is lack of in-depth research.in most normal development, such as the growth of the primary intestine, the development of the neural tube (the neural crest development), the migrated cells must be connected to specific cells in a new location.these new connections are formed, they must be able to keep together steadily during development.because during development, morphogenesis occurs with a strong thrust and a change in pull.in cancer metastasis, the establishment of a new connection between cells and cells is also a step in the way.However, this important process is also difficult in the in vivo study because it is difficult to know in advance what new location the cancer cells will transfer to., denise J. Montell of the University of California, Santa Barbara (first author, Dr. Miao Guangxia) published an article in the development al Cell journal, Integration of Migratory Cells into a New Site In Vivo Vieo Requires Channel-Independent Function Sinnexins on Microtubules, using the black belly fruit fly (Drosophila melnosy.fruit fly's ovaries are made up of egg chambers at different stages of development.each egg chamber is encased in a single layer of folcle cells (15 nurse cells) and one egg cell (eggyte).during ovarian development, six to eight boundary cells are stratified from follicle cells and begin to migrate to the boundaries of the egg cells.when boundary cells reach the boundaries of the egg cells, they make morphological changes and attach to the egg cells.at the same time, centripetal cells (a layer of follicle cells between the egg cells and the helper cells) also begin to migrate from the edge of the egg chamber to the center.as the centripetal cells migrate, the heart cells and the boundary cells meet and form new connections, eventually forming the structure of the micropyle (Figure 1). sperm enters the egg cells through the hole to form a fertilized egg. In this study, the researchers first described how border cells attached to the egg cells after the migration, and how to establish a new connection with the centripetal cells, using the results of fixed samples and living images. this is the first time that there has been a study in the body, from the tissue and cell level sinforth to detail the process of establishing a new connection between cells and cells after cell migration is completed. further, through RNA interference techniques, it was found that lowering Innexins in boundary cells affects the formation of new connections between boundary cells and egg cells. Innexins is a family of proteins that form gap junctions and functional analogs (functional analogs) of vertebrate-connected proteins (Connexin). Innexins is a family of four-times-penetrating membrane proteins, eight of which form a semi-pathway (hemichannel) on the cell membrane, forming a gap connection in the semi-pathway between the two cells. small molecules, such as calcium ions, can be transferred from one cell to another through a gap connection. researchers found that downgrading Inx2 and Inx3 did not affect the completion of migration of boundary cells, but the boundary cells did not adhere steadily to the egg cells after full migration. the results of experiments such as immunostaining, penetrating electroscopy and thermal genetics, it was found that Innexins' channel independent function played an important role in regulating this process, while the classic gap connection function (gap junction channel) was not important in this process. researchers also found that Innexin 2 and innexin 3 are in boundary cells, where innexin 4 regulates the stability of microtubesin , which in turn allows boundary cells to adhere steadily to the egg cells. This is the first study in the body to reveal the molecular mechanisms of a new connection between cells. in this , the researchers revealed the important functions of Innexins and microtube proteins in establishing new connections between cells and cells through experimental results such as fruit fly genetics, immunostaining, active target imagery, thermal genetics, and penetrating electrosorics. this paper establishes the mechanism of establishing a new connection between cells and cells based on the boundary cells of fruit flies, and for the first time clarifies the mechanism of cell-cell-cell-to-cell-building. this study will help people to better understand the mechanisms of cell migration and cell migration, and is of great significance for the study of normal developmental processes and cancer metastasis mechanisms. .