Determination of polyphenols in water soluble extract of Cornus by Folin ciocalteu method
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Last Update: 2015-03-05
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Source: Internet
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Author: User
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Recently, researchers from the Medical College of Henan University of science and technology published a paper to study the optimal conditions of Folin ciocalteu method for the determination of polyphenols in the water-soluble extract of Cornus officinalis The results showed that Folin ciocalteu method was sensitive, accurate, reliable, stable, simple and easy to use under the optimized conditions It was suitable for the determination of polyphenols in the water-soluble extract of Cornus This article was published in the 2nd issue of Journal of Henan University of science and Technology (Medical Edition) in 2014 Taking absorbance as evaluation index, the effects of detection wavelength, Folin ciocalteu reagent dosage, Na2CO3 dosage, reaction temperature and reaction time on the determination results were studied The optimum conditions were as follows: the detection wavelength was 760 nm, 0.4 ml of sample was added with 1.0 ml of Folin ciocalteu reagent and 3.2 ml of 10% Na2CO3 solution, the reaction temperature was 35 ℃, the reaction time was 1.0 h, and the relationship between absorbance and polyphenol content was good in the range of 0-250 μ g / ml (y = 0.0053x + 0.0389, R2 = 0.9993) The recovery was 98.5% - 103.0%, and the RSD was 1.9% - 2.3% (n = 6) The average content of polyphenols in the water-soluble extracts of three batches of Cornus was 39.24%.
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