Delta is not flat, and Lambda rises again to see how gene sequencing is "eye-catching" to monitor mutations!

Rapid transmission, strong infectivity, short incubation period, high viral load, atypical symptoms, long treatment time.

If you pay attention to the news of epidemic prevention and control, it is not difficult to find that when reporting the latest situation of epidemic prevention and control, all places will also announce the epidemic situation and traceability

High-throughput sequencing is an important technical means for the traceability of the new coronavirus

The "New Coronavirus Pneumonia Prevention and Control Plan (Eighth Edition)" document pointed out: the first or early cases in the local epidemic, the key cases that are epidemiologically related to the early cases, the local cases with unknown sources of infection, and overseas imported cases , Inbound articles and related environmental positive specimens carry out virus gene sequence determination and comparison analysis, dynamically understand the virus gene mutation situation, and timely find the source of infection

Due to the feature that it does not rely on microbial culture and can directly sequence nucleic acids in clinical samples, NGS (High Throughput Sequencing) technology has become an important technical means for tracing the source of new coronaviruses

In our previous news, " Mutated new coronaviruses were discovered in time in many countries, and gene sequencing is indispensable!" In ", the application of high-throughput sequencing in the research of new coronaviruses is introduced in detail, and interested friends can click to view!

Since the outbreak of the new crown epidemic, mutant strains of the new crown have emerged one after another, the "delta" has not yet settled, and the "lamda" with a higher viral load has risen again! In order to better understand the dangers posed by these mutant strains, scientists have been tracking and monitoring the frequency and spread of virus mutations

Researchers such as Tanya Golubchik, University of Oxford, UK, collaborated to reveal the diversity and spread of SARS-CoV-2 in the host

Researchers found that most patients with COVID-19 carry only one or two mutated strains of COVID-19, and most of them cannot survive transmission to others.

Further studies have shown that the probability of a variant strain with enhanced transmission or immune escape is very low, but once it is successfully transmitted, it may spread rapidly

At the same time, the article pointed out that because escape mutations may be very adaptive in people with high vaccination rates or high levels of natural immunity, and the mutation effect may depend on their genetic background, continuous attention and monitoring are very necessary

Takara's new coronavirus whole genome sequencing solution

When using clinical samples such as nasal swabs, throat swabs, and alveolar lavage fluid for virus NGS analysis, problems such as low virus content, complex sample background, and uneven PCR amplification during database construction are often encountered, so that the virus can be analyzed correctly.

SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (Pico v2, Code No.

▪250 pg-10 ng of total RNA starting amount, and the library can be built efficiently with few samples!

SMARTer Stranded Total RNA-Seq Kit v3-Pico Input Mammalian (Pico v3, Code No.

▪ By adding UMI , it can more sensitively identify PCR repeated fragments and correct sequencing errors!

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For more details, please click:
Takara Bio China official website