Defecation effect of water extract from calyx of Roselle

2015-12-27 classification: efficacy and action: one person reviews the literature and reports that Hibiscus sabdariffa can be used as diuretic to treat hypertension, and has antibacterial activity This paper further evaluated the purgative and laxative effects of the water extract from the calyx of new Roselle flowers Experimental materials: soak the fresh and air dried calyx of Roselle in 500g hot water for 1 hour, filter and freeze dry the soaking solution to obtain 12g plant extract, and store it at 4C for standby The preparation of senna leaf extract is the same as above Methods: Experiment 1: change normal defecation The experiment was divided into three groups: group A, B and C, 10 rats in each group, 1 rat in each cage Group A was orally given 0.1ml distilled water containing 800mg / kg freeze-dried plant extract and 1% carmine; group B was orally given the same dose of senna leaf and dye; group C was only given 1.0ml distilled water containing dye To detect the average (past) time between administration and first defecation of the dye Experiment 2: purgative test Twenty rats were randomly divided into two groups They were given 400 and 800 mg / kg Roselle extract by mouth, senna extract by positive control group and 0.1 ml distilled water by negative control group Fecal excretion was measured 12 hours later Experiment 3: small intestine transport Take 10 adult mice and give 800mg / kg extract orally for 24 hours before the experiment, and then give 1.0ml suspension containing 5% activated carbon and 10% tragacanth gum to each mouse Kill the animals after 5 minutes, open the abdomen, take out the small intestine, calculate the fecal transport rate through the transport juice of activated carbon, and calculate the positive control group (give senna extract) and negative control group as well The small intestine transport rate of group B (treated with distilled water) Results: ① Roselle extract had no effect on defecation of normal rats, the results were the same as that of the control group, but senna extract could promote defecation, which was significant compared with the negative control group ② Rose eggplant extracts (400 and 800 mg / kg) showed a moderate effect on defecation, and wet stool increased significantly ⑧ Roselle extract did not significantly enhance intestinal peristalsis, but there was significant difference between senna leaf and negative control group The results suggested that the purgative effect of Roselle extract might be caused by saponin like compounds in the extract.