Cytokine: CXCL12 promotes the proliferation of radioactive glial cells after brain trauma in rats.

The study, which explores the effects of CXCL12 on radioactive glial cell regeneration after brain trauma (TBI), was published online in Cytokine.
48 rats were randomly divided into 4 groups: (1) false group, rats only had open-cranial surgery; (2) control group, TBI after the same side cortical injection physiological saline; (3) CXCL12 group, injection CXCL12 and AMD3100 group, injection CXCL12 and AMD3100.
7 days after TBI, brain tissue was immune-fluorescent double-label staining of BdU/Nestin, BLBP/Nestin, BLBP/Vimentin, BLBP/SOX2, BLBP/CXCR4, BLBP/DCX.
we use Western Blot to determine the level of Nestin, BLBP, and Vimentin.
results showed that CXCL12 treatment significantly increased the number of cells stained around the injured cortical and cytosome areas of BdU/Nestin, BLBP/Nestin, and BLBP/Vimentin compared to the control group.
the number of these cells decreased significantly compared to the CXCL12-AMD3100 treatment compared to the CXCL12 treatment and control group.
levels of Nestin, BLBP and Vimentin were the same as between immunofluorescent staining.
BLBP/Vimentin-positive cells show star cell patterns around the injured cortical region, but RGCsaround the injured carcellular region.
BLBP-positive cells also express CXCR4 and SOX2.
, CXCL12 promotes the proliferation of nerve prescient cells after TBI by combing through its subject CXCR4.
cells that multiply in the united appear radial glial cell-like cells.
RGCs-like cells can differentiate into immature neurons, facilitating the migration of immature neurons.
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