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    Home > Active Ingredient News > Drugs Articles > Cultivation of Arabidopsis thaliana in an artificial climate chamber

    Cultivation of Arabidopsis thaliana in an artificial climate chamber

    • Last Update: 2022-09-09
    • Source: Internet
    • Author: User
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    Arabidopsis thaliana is a cruciferous plant widely used in the research of plant genetics, developmental biology and molecular biology, and has become a typical model plant
    .

    For this typical plant, Zhe Tu will lead you to analyze the cultivation of Arabidopsis in an artificial climate box
    .

    , to know what conditions are required for the growth of Arabidopsis thaliana? 1.
    Temperature The ideal temperature range of the Arabidopsis growth chamber is 16-25°C, and the optimal growth temperature is 22-23°C
    .

    If the temperature is too high (above 28°C), the flowering and fruiting will be affected.
    Under the condition of sufficient water, it can endure the high temperature of 34°C
    .

    Arabidopsis grows fast when the temperature is high, and grows slowly when the temperature is low.
    Therefore, the growth rate of Arabidopsis can be adjusted by selecting different culture temperatures according to the experimental purpose and schedule
    .

    2.
    The relative humidity of the air Arabidopsis likes to be humid.
    When the relative humidity of the growth environment is relatively high, Arabidopsis can grow well.
    Otherwise, the fruiting is less, the plants are thin, and the leaves are easy to dry up
    .

    When cultured at 22°C, the relative humidity should be kept above 70% and below 80%, and should not be lower than 50%
    .

    But higher relative humidity (>90%) can cause infertility
    .

       3.
    Lighting The optimal light intensity of Arabidopsis growth room is about 120-150μmol/m2/s
    .

    Generally, 16h light and 8h dark photoperiod are used to cultivate Arabidopsis thaliana
    .

    Light more than 12h, will promote reproduction, and vice versa to promote plant tissue growth
    .

    Photoperiod can also be used to regulate the growth of Arabidopsis thaliana.
    For example, under the short light conditions of 8 h light and 16 h dark, Arabidopsis thaliana vegetative growth period is prolonged, flowering is late (about 8 weeks), but nutrient accumulation is high
    .

       4.
    Arabidopsis growth chamber The seeds were placed in a high humidity environment at 4°C for 2-4 days for vernalization
    .

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