CRISPR technology can study early embryonic development in humans

CRISPR-Cas9
genome editing technique has been used to study the role of the OCT4 gene in early human embryonic development, according to a paper published in the British Journal of Science and Technology. The results provide a framework for future research and provide new insights into the molecular mechanisms that control embryonic development. This principle study suggests that CRISPR-Cas9 genome editing techniques can be used to assess the role of genes in early human development.
In early human development, fertilized eggs are differentiated into blastocysts, which contain 200 to 300 cells with different functions: some are called pl potent outer embryo cells, which continue to develop into fetuses; others are called extraterital cells, which form placental and spalk sacs. Unfortunately, the mechanisms that determine the fate of these early cells are still unclear. Although the OCT4 gene is considered a gene necessary for erythration and reprogramming in human cells, its function in early human embryos is still little known.
Casey Niaken of the Francis Crick Institute in the United Kingdom and colleagues, used an optimized CRISPR-Cas9 genome editing method to target the editing of the OCT4 gene in human fertilized eggs. The study used donated excess human fertilized eggs used to treat infertility.
that the OCT4 gene is essential in early human development and that when its expression is inhibited, human development is affected. They also found the role of OCT4 in regulating the expression of genes involved in extratertotal cells and the expression of errative genes.
authors say their results set the stage for future research that promises to further enhance understanding of human embryonic development and improve INSE therapy, ultimately contributing to improved stem cell development and treatment applications. (Source: Science and Technology Daily Zhang Mengran)