Cordyceps sinensis has significant immunoregulatory effect

In 1990, Zhu Xiyuan et al Published the first article on the immunosuppression of Cordyceps sinensis on cell immunity in volume 10, issue 8 of the Journal of integrated traditional Chinese and Western medicine The experimental sample was provided by the author The cell phagocytic function of mice whole blood with yeast polysaccharide was measured by chemiluminescence method, which was in the range of 0.6-5.0mg/ml At the same time, lymphocyte transformation at 0.6-5.0mg/ml and two-way mixed lymphocyte culture at 0.31-5.0mg/ml had a significant inhibitory effect and a dose-response relationship It is proved that Cordyceps sinensis has no cytotoxicity In 0.25-5.0mg/ml Cordyceps sinensis, the secretion of IL-1 induced by LPS of E There was no significant difference between the two groups In 1993, Meng Ming et al Published the title of immunosuppressive function of Cordyceps sinensis extract in Volume 13, issue 5 of Shanghai Journal of immunology The sample was 60% alcohol extract of Cordyceps sinensis, 1.5-12g was administered intraperitoneally/ Kg? D-1? D ~ 7d inhibited the proliferation of ConA and LPS, the formation of antibody secreting cells, the delayed hypersensitivity of alloantigens and the rejection of allografts The crude product, water extract and 60% alcohol extract of Cordyceps sinensis had the same inhibition About the immunosuppressive mechanism of Cordyceps sinensis Most scholars think that Cordyceps sinensis acts on T cells and selectively increases TS cells, and one of its mechanisms is to play the negative feedback regulation of immune response The antibiotic ISP-1 (myriocin / thermozymocidin), which was extracted from the culture medium of Isaria sinclairi, was chemically modified and synthesized Its chemical structure and mechanism of action are different from the existing immunosuppressants It acts directly on lymphocytes and shows immunosuppressive effect In particular, the inhibition of rejection of organ transplantation is particularly noticeable In all kinds of animal experiments of organ transplantation and skin transplantation, the rejection showed good inhibition or treatment effect, and no side effects occurred at the same time Moreover, the immunosuppressive effect is better when the immunosuppressive agent is used simultaneously with the existing immunosuppressive agent All kinds of fungi of Ascomycetes, which can parasitize on insects, may have anti rejection substances, which can make them resist the host's immune attack and make their mycelia proliferate The significance of the discovery and isolation of new immunosuppressant FTY720 is to prove this It provides a new way for us to find new immunosuppressants The following three abstracts of Cordyceps sinensis in the pharmacological study of immunosuppression are as follows In this paper, th, TS and th / Ts ratio were measured, and Th 1 was measured It was judged that the immunosuppressive effect of Cordyceps sinensis was through increasing the number of T lymphocytes (the previous article believed that Cordyceps sinensis has enhanced the cellular immune function), but in the increased T lymphocytes, the number of th and TS increased, but the number was not uniform, the number of th increased less, and the number of TS increased more As a result, th / TS increased more In th, there are Th1 and Th2 cells Cordyceps sinensis can reduce Th1 and immunosuppression This is the immunosuppressive mechanism of Cordyceps sinensis Wang Xudan et al Reported that 5-10g / kg of Cordyceps sinensis water decoction can significantly inhibit the immune function, which is consistent with the dose mentioned in Section 2 of this chapter 1 The effect of Cordyceps sinensis on the immune function of mice [1] [research methods] natural Cordyceps sinensis in Naqu, Tibet was selected Decoct twice with distilled water, combine the filtrate twice, concentrate to 50% in water bath, and dilute according to the required dosage BALB / C pure strain mouse, female, body weight (20?) g There were 4 dose groups of Cordyceps sinensis, namely 0.5g/kg, 1g / kg, 5g / kg, 10g / kg Each group was given 0.4ml/animal by gavage for 10 days Test items: the effect of Cordyceps sinensis on the proliferation of mouse spleen cells, the one-way mixed lymphocyte culture, the induction and activity of IL-2, the induction and activity of IL-1, the activity of NK cells [results] 1 The proliferation of ConA (P < 0.001) in mouse spleen cells was significantly inhibited by the administration of 5-10g / kg of Cordyceps sinensis water decoction, the proliferation of LPS (P < 0.01) was inhibited by the administration of 1G / kg of Cordyceps sinensis water decoction, and the proliferation of LPS (P < 0.001) was inhibited by the administration of 5-10g / kg of Cordyceps sinensis water decoction; 2 The proliferation of MLR (P < 0.001) was inhibited by the administration of 5-10g / kg of Cordyceps sinensis water decoction < 0.05) and the synthesis of IL-1 and IL-2 (P < 0.01) With the increase of the dosage of Cordyceps sinensis, its inhibition effect is more obvious However, 0.5-10g/kg of Cordyceps Sinensis Decoction significantly enhanced the activity of NK cells (P < 0.001) It should be noted that there is no dose-response relationship between the enhancement effect of 0.5-10g/kg The effect of Cordyceps sinensis on immune function is various, which may be related to its different effects on different immune effector cells It has a significant dose-dependent inhibitory effect on lymphocyte transformation, MLR, IL-1, IL-2 and other immune responses in mouse spleen The inhibition of IL-1 secretion by Cordyceps sinensis may be another mechanism of its inhibition of immune response Cordyceps sinensis can significantly enhance the activity of NK cells from low dose to high dose, and there is no correlation between the enhanced effect and drug dose In the cases of organ transplantation and autoimmune diseases, Cordyceps sinensis as an assistant treatment will have a good prospect At the same time, according to the different doses of Cordyceps on the immune system, the appropriate dose can be selected according to the actual situation 2 Immunosuppressive effect of Cordyceps sinensis extract [2] Cordyceps sinensis crude product, water extract and alcohol extract were injected intraperitoneally for 4 consecutive days to inhibit the proliferation of ConA and LPS, which were similar to each other; to inhibit the IgM antibody secreting cells (PFC) of mouse spleen, water extract was stronger than the other two; to DTH three similar, water extract was slightly stronger than the other two; to allogeneic skin transplantation crude extract and water extract p The weight of spleen and the total number of spleen lymphocytes remained unchanged, T lymphocytes increased, the number of th and TS and the total number increased P < 0.01, the ratio of th / TS increased P < 0.01 It is shown that Cordyceps sinensis plays an immunosuppressive role by regulating Th / Ts ratio 3 The effect of Cordyceps sinensis on the expression of PCNA and IL-2 in the mixed lymphocyte culture of mice [research methods] the medicine was ground into powder, soaked overnight in water, concentrated into a decoction containing 0.25g/ml crude medicine with mild fire, and the impurities and microorganisms were removed with 0.45M pore size filter membrane Cyclosporine A was mixed into 0.5 μ / ml solution Mixed culture was carried out with reactive lymphocytes and stimulating lymphocytes and corresponding drugs were added 40 / hole Proliferating cell nuclear antigen (PCNA) and IL-2 were measured in mixed lymphocyte culture of mice [results] 1 PCNA is a good indicator of cell proliferation: the expression of PCNA in Cordyceps sinensis group was significantly lower than that in the control group without drugs, but lower than that in cyclosporine a group, indicating that it can inhibit the proliferation of lymphocytes, but the effect is weaker than cyclosporine A 2 IL-2 is produced by activated Th1 cells, which can cause T cell proliferation, produce lymphokines, activate natural killer cells (NK) and up regulate immune rejection IL-2 is the most important cytokine involved in immune rejection The results showed that it could reduce the expression of IL-2 in mixed lymphocyte culture, indicating that Cordyceps sinensis could down regulate the cellular immune response by inhibiting the release of Th1 cytokines 3 Cordyceps has two-way immunoregulation on peripheral blood lymphocytes It can be seen from the results of this experiment that the dosage of the two drugs in the compound preparation group can be reduced to 1 / 2 of that in the single drug group, while the effect on the expression of PCNA and IL-2 is basically the same as that in the single drug group It is proved that the compound preparation can reduce the dosage of Begonia, play the role of immunosuppression and reduce the rejection, and at the same time, reduce the toxic and side effects in vivo, but its mechanism remains to be explored Further study Many experimental results show that there is no significant difference in composition and function between natural Cordyceps sinensis and Artificial Cordyceps sinensis Ye Jia, chief executive of nacqu Cordyceps, said that the development of Artificial Cordyceps will be the industry trend.