Complete the mapping of whole genome sequence map of sweet orange

The reporter recently learned from Huazhong Agricultural University that after more than one year's research, researchers in the fields of horticulture, genomics, bioinformatics, etc have completed the splicing and annotation of sweet orange genome and obtained high quality sweet orange genome This is the first genome sequence map of fruit tree crops independently completed in China Citrus is one of the most important fruit trees in the world Sweet orange is the world's largest type of citrus, accounting for about 60% of the world's citrus production, and orange juice is one of the world's three major drinks However, the development of genetics and breeding of sweet orange is hindered because of its characteristics of multiple embryo, male or / and female sterility, high heterozygosity of genetic background and so on Complete genomic information, as a genetic framework, will provide basis for citrus genetic and breeding improvement It is reported that there are 9 pairs of chromosomes and the genome size of sweet orange is 367M The whole genome shotgun method (WGS) was used to sequence the genome of sweet orange The genome annotation combined abundant gene expression data, including transcriptome of callus, leaf, flower and fruit Rna-pet technology was introduced to annotate the gene transcripts, and about 30000 genes were annotated The genome sequence was integrated into 9 chromosomes by genetic markers and in situ hybridization By comparison, the common genes of sweet orange, grape and strawberry were 81% and 83%, respectively It was also found that 51% of the genes were heterozygous China is an important primary center of Citrus in the world, with rich types of cultivated citrus, which can be divided into citrus, orange, orange, grapefruit, lemon, kumquat, citron and ornamental four season orange In 2010, China's total output was 26.45 million tons, with a planting area of 2.21 million hectares, making it the world's largest citrus production country According to academician Deng xiuxin, President of Huazhong Agricultural University and a famous citrus expert, the completion of the sweet orange genome will provide an important platform for colleagues at home and abroad to study the special biological characteristics and functional genome of Rutaceae (Citrus) At the same time, it is of great significance for the discovery and variety improvement of the genes of important characters such as fruit color, aroma, taste, maturity, etc., indicating that the citrus base in China It is of great significance for promoting the competitiveness of the industry to make basic research in the forefront of the world.