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Cell culture is widely used in scientific research and biopharmaceuticals.
To date, there have been a variety of mycoplasma detection methods, including culture methods, indicator cell methods, nucleic acid amplification (NAT)-based methods, and enzymatic reaction-based methods (biochemical methods), each of which has its own characteristics and applications.
Biochemical method is a detection method based on the biochemical activity of mycoplasma, and related kits can detect mycoplasma enzymes, such as acetate kinase and carbamate kinase
The principle of biochemical detection:
Table 1 Comparison of three common mycoplasma detection methods
The paper titled "Sensitivity of biochemical test in comparison with other methods for the detection of mycoplasma contamination in human and animal cell linesstored in the National Cell Bank of Iran" used culture, biochemical methods (LonzaMycoAlert® kit) and PCR to analyze the large number of cell banks.
In this study, the researchers randomly selected 40 different human and animal cell lines, and used Vero and NSO cell lines as positive and negative controls, respectively, for mycoplasma detection using culture, biochemical and PCR methods
Table 2 Test results of three methods
Figure 1.
The biochemical method kit MycoAlert used in the research is from Lonza.
Figure 2.
