Comparison of antioxidative effect between black Wolfberry Extract and red Wolfberry Extract

Black wolfberry is a kind of wolfberry plant in Solanaceae, which grows in desert and Gobi It has many functions such as eye protection, beauty care, immune regulation, anti-aging, improving blood circulation, preventing arteriosclerosis and so on, so it enjoys the reputation of "soft gold" Red wolfberry is rich in wolfberry polysaccharide, vitamins, minerals, β - carotene and other nutrients, which can significantly improve the activity of superoxide dismutase (SOD), fight against free radical peroxidation, and help to eliminate the active oxygen, thus helping to delay aging and anti fatigue As a natural wild plant, Lycium barbarum is rich in free amino acids, minerals, alkaloids, vitamins and other essential nutrients for human body The content of free amino acids and microelements in Lycium barbarum is much higher than that in Lycium barbarum, which is more conducive to regulating the immune function of the body In addition, black wolfberry contains a lot of anthocyanins, which is not available in red wolfberry Anthocyanin is the most effective natural antioxidant, which has stronger ability of scavenging free radicals than vitamin C The anthocyanin, polyphenol, polysaccharide and other active ingredients in black wolfberry can delay the aging of human cell tissue, remove oxygen free radicals, keep the body young, enhance immunity and prolong life People pay more and more attention to the efficacy of black wolfberry Compared with red wolfberry, black wolfberry has greater research value and broader market prospects This experiment not only confirmed that the extract of black wolfberry has a greater role in scavenging free radicals than the extract of red wolfberry, but also fully extracted the active ingredients of black wolfberry by combining water extraction, alcohol extraction and ultrasonic technology, providing reference for the application of black wolfberry in the fields of food, health products and cosmetics 1 material 1 1 main materials: Black wolfberry stem, red wolfberry stem (Qinghai), anhydrous ethanol (Nanjing Chemical Reagent Co., Ltd.), DPPH (sigma, USA) 1.2 instruments: ultrasonic cleaning machine (Shanghai Precision Scientific Instrument Co., Ltd.), vacuum freeze dryer (Shanghai Liwen Scientific Instrument Co., Ltd.), ultraviolet visible spectrophotometer (Unocal (Shanghai) Instrument Co., Ltd.), high-speed freeze centrifuge (Shanghai luxianyi centrifuge instrument Co., Ltd.), grinder (Zhejiang new century crushing equipment Co., Ltd.) 2 method 2.1 the process of preparing black wolfberry and red wolfberry water extract is shown in Figure 1 Black wolfberry dry / red wolfberry dry → smash → distilled water → repeat extraction three times → combine extract → centrifugation → take supernatant → freeze-drying → black wolfberry / red wolfberry water extract 2.2 the process of preparing black wolfberry and red wolfberry ultrasonic water extract is shown in Figure 2 Fig 2 preparation process of ultrasonic water extract of black wolfberry and red wolfberry 2.3 preparation process of alcohol extract of black wolfberry and red wolfberry is shown in Fig 3 Dried black wolfberry / dried red wolfberry → crushed → anhydrous ethanol → repeated extraction for three times → combined extract → centrifugation → supernatant extraction → freeze-drying → ethanol extract of black wolfberry / red wolfberry Fig 3 preparation process of ethanol extract of black wolfberry and red wolfberry Fig 2.4 preparation process of ultrasonic ethanol extract of black wolfberry and red wolfberry Fig 4 It is shown Fig 4 preparation process of ultrasonic alcohol extract of black wolfberry and red wolfberry 2.5 DPPH free radical scavenging test Dissolve the weighed DPPH powder with anhydrous ethanol and fix volume, prepare DPPH solution and store in dark The extracts of black wolfberry and red wolfberry were prepared into 0.2, 0.4, 0.6, 0.8 and 1.0 mg / ml solutions respectively Take 2.0 ml of sample solution of each concentration, add 2 m ldpph solution respectively, place in dark for 30 min, use 2 ml absolute ethanol instead of DPPH solution as blank group, use distilled water of equal volume as control group instead of sample, use ultraviolet spectrophotometer at 517 nm The DPPH radical scavenging rate was calculated according to the formula The results of clearance rate (%) = {1 - [(AI AJ) / A0]} × 100% 3 and analysis of the effect of water extract on DPPH free radical scavenging ability 3.1 The comparison of DPPH free radical scavenging ability of different concentrations of black wolfberry water extract and red wolfberry water extract is shown in figure 5 The water extract of black wolfberry has good free radical scavenging ability from the beginning of low concentration, while the free radical scavenging ability is very low when the concentration of water extract of red wolfberry is low When the concentration of extract reaches 1 mg / ml, the scavenging ability is still far lower than that of water extract of black wolfberry 3.2 effect of alcohol extract on DPPH free radical scavenging capacity comparison of DPPH free radical scavenging capacity of different concentrations of black wolfberry alcohol extract and red wolfberry alcohol extract is shown in Figure 6 The free radical scavenging ability of the alcohol extract of black wolfberry was significantly higher than that of the alcohol extract of red wolfberry, and the highest scavenging ability was 55% 3.3 effect of ultrasonic water extract and ultrasonic alcohol extract on DPPH free radical scavenging ability Ultrasonic technology was used as auxiliary technology to extract the active components from black wolfberry and red wolfberry respectively by water extraction and alcohol extraction, and DPPH was compared On the basis of the scavenging ability of free radicals, the antioxidant capacity of the extracts obtained by different extraction methods was further compared It can be seen from Figure 7 that the free radical scavenging ability of ultrasonic water extract and ultrasonic alcohol extract of red wolfberry is significantly lower than that of black Wolfberry Extract, and the increase of the concentration of red Wolfberry Extract has little effect on the free radical scavenging ability, but in a certain range, the effect of black Wolfberry Extract on the DPPH free radical scavenging ability is relatively large, and the scavenging ability increases with the increase of the concentration The order of several extracts was: ultrasonic water extract of black wolfberry > ultrasonic alcohol extract of black wolfberry > ultrasonic water extract of red wolfberry > ultrasonic alcohol extract of red wolfberry The free radical scavenging ability of ultrasonic water extract of Lycium barbarum was the strongest, up to 87.6% Conclusion as far as Lycium barbarum is concerned, the main antioxidants are Lycium barbarum polysaccharide and β - carotene As for Lycium barbarum, anthocyanin is the main antioxidant Anthocyanins can also prevent cardiovascular disease and arteriosclerosis Red wolfberry does not contain anthocyanin Black wolfberry is the fruit with the highest anthocyanin content in nature Compared with red wolfberry, black wolfberry contains more alkaloids and trace elements It can be seen from DPPH free radical scavenging ability experiment that compared with red Wolfberry Extract, black Wolfberry Extract has better antioxidant, scavenging free radicals and anti-aging effects It has a broad prospect to develop cosmetics, medicine, health care products and so on Source: comparison of antioxidant effect between black Wolfberry Extract and red Wolfberry Extract Yang Fengqi, Hu Tiejun, etc you may also want to see the nutritional and health care effect overview of black wolfberry Comparison between black wolfberry and red wolfberry Research progress and application prospect of black wolfberry Research progress of basic theory and value of black wolfberry The present situation and Prospect of the development of the characteristic products of black wolfberry