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Chinese Academy of Agricultural Sciences: The combination of transcription factor MdWRKY32 and MdBam5 promoter participates in starch-sugar metabolism during apple storage

 Last Update: 2021-12-24
 Source: Internet
 Author: User

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Apple is an important planting fruit tree in northern China, and its output and export volume rank first in the world

1) The effect of different treatments on the physiological indexes of apples after harvest

This study measured the weight loss rate, hardness, respiration rate and ethylene release rate of apple fruits during postharvest storage under different temperatures, ethylene and 1-MCP treatments

Starch degradation is a sign of postharvest maturity and contributes to the accumulation of soluble sugar in apple fruits

The gene expression of 5 members ( MdBam5 , MdBam6 , MdBam8 , MdBam14, and MdBam19 ) quickly responded to exogenous ethylene treatment induction and was inhibited by 1-MCP treatment.

4) Instantaneous overexpression score of MdBam5

"Wang Lin" apple pulp has high permeability, and the pulp contains a lot of starch to facilitate staining observation, so we use "Wang Lin" pulp transient overexpression experiment to verify the function of MdBam5 gene

5) The MdWRKY32 transcription factor binds to the cis-acting element of the MdBam5 promoter

The cis-acting element of the MdBam5 promoter, which is a key β -amylase gene family member in the apple’s postharvest starch degradation, was identified through Plant-Care website analysis .

In order to further confirm the effect of MdWRKY32 on starch degradation, its full-length CDS sequence was constructed on the SK vector and overexpressed in common tobacco leaves

Corresponding author Jane

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