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Canola (Brassica napus L.)

 Last Update: 2020-11-11
 Source: Internet
 Author: User

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transformation efficiency formula

frequency increases wavelength

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High-frequency
Agrobacterium tumefaciens
-mediated transformation can be obtained in canola by optimizing the preconditioning time of the explant and cocultivation time
with A. tumefaciens
. A preconditioning time of 72 h and cocultivation of 48 h synergistically increase the transformation efficiency to 25%. In addition, the recovery of transgenic plants can be facilitated by overcoming hyperhydration, which increases the rooting frequency to 100%.

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