Building a model of glioblastoma organs

There was no statistical difference in the rate of growth of GBM in the brains of young and older organ brainsHowever, gbM growth patterns varied in the brains of organs of different ages, and gliomas in the brains of 4-month-old organs were more leaching- Excerpted from the article.Ref: Linkous A , et alCell Rep2019 Mar 19;26 (12):3203-3211.e5doi: 10.1016/j.celrep.2019.02.063tumor organoid is grown from tumor cells in three dimensions, closer to tumor characteristics than the traditional culture of tumor cells, and is a commonly used method for screening drug cultureAt present, scientists have successfully established organ models for malignant tumors such as liver cancer and breast cancer, but the culture of glioblastoma (GBM) organ culture is relatively difficult and less research has been madeAmanda Linkous of the Maya Cancer Center at Wilcoyer Medical School in New York, among others, used glioma stem cells (GSC) and human embryonic stem cells (hESC) in patients to develop a new model of GBM organoid glioma, which was published in Cell Reports in March 2019to accurately simulate GBM, the authors cultured GFP-labeled GSC symbiosis for 24 hours with individually established organ brains, and all GSC-derived gliomas were intruded into brain organsTumor growth was detected in brain organs after 1 week of culture, and 20% of tumor cells were positive for Ki-67 stainingThe morphological and pathological characteristics of these newborn tumors are very close to surgical specimens (Figure 1)Figure 1GSC forms invasive tumors in brain-like organsthe microtubal network of tumors provides a way for tumor cells to attack and multiply, and promotes communication between tumor cellsThe authors also observed similar microtube networks on GBM growing in the brain of organ-like organs Electron microscope photos show that cell interstitials and intracytoplasmic connecting protein-43 positive expression, tumor microtubulas can effectively transmit calcium signals Therefore, the glioma organ can be used for in vitro research of microtubuy tubes (Figure 2) Figure 2 The growth of glioma-type organs depends on tumor microtubule subsequently, the authors examined the sensitivity of glioma-like organs to chemotherapy and radiotherapy The performance of glioma-like organs after 5Gy radiation exposure was basically similar to that of the control group; Figure 3 Different responses of glioma-type organs to treatment authors found no statistical difference in the rate of growth of GBM in young and older organ brains However, there are differences in GBM growth patterns in the brains of organs of different ages; gliomas in the brains of 4-month-old organs are more immersive (Figure 4) Figure 4 The growth rate of glioma-type organs is similar , the glioma model of the organ-like brain established by the authors shows that GSC can invade and multiply in the organ-like brain, forming tumors close to the patient's GBM The glioma organ can be a powerful tool for modeling and high-throughput drug screening for in vitro primary generation GBM.