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Blueberry (Vaccinium corymbosum L.)

 Last Update: 2021-03-13
 Source: Internet
 Author: User

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Recent advances in plant biotechnology have led to a reliable and reproductive method for genetic transformation of blueberry. These efforts built on previous attempts at transient and stable transformation of blueberry that demonstrated the potential of
Agrobacterium tumefaciens
-mediated transformation, and as well, the difficulties of selecting and regenerating transgenic plants. As a prerequisite for successful stable transformation, efficient regeneration systems were required despite many reports on factors controlling shoot regeneration from leaf explants. The
A. tumefaciens
-mediated transformation protocol described in this chapter is based on combining efficient regeneration methods and the results of
A. tumefaciens
-mediated transient transformation studies to optimize selected parameters for gene transfer. The protocol has led to successful regeneration of transgenic plants of four commercially important highbush blueberry cultivars.

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