Blood: Researchers of normal and malignant hematopoietic stem cells OF SIRPA knock into model
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Last Update: 2020-06-22
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Source: Internet
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Author: User
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In human-mouse xenotransplantation, the polymorphism of signal-regulating protein alpha (SIRPA) determines its binding affinity with human CD47, which is essential to the implantefficiency of human cellsIn this study, researchers established a new C57BL/6Rag2null Il2rgnull (BRG)mouse line through Sirpahuman/human (BRGShuman), in which the Sirpa gene was replaced with the human SIRPA gene for the entire (eight exons)the mice's macrophages had significantly stronger affinity for human CD47 than SirpaNOD/NOD, and did not show detectable phagocytosis against human hematopoietic stem cellsin turn, the macrophagesof the of the of themice had only moderate affinity against CD47 in mice, and BRGShumanmice did not exhibit blood cell reduction in the-/-mice in xenotransplantation, BRGS human mice showed a highly effective transplant, maintained human hematopoietic function, had a high level of myelin cell reconstruction, and improved reconstruction of surrounding tissues compared to the BRG in the mice of Sirpa NOD/NOD (BRGSNOD) BrGS human mice also showed significant increases in implantation and growth of human colon cancer cells in human colon cancer cells with acute myeloid leukemia and subcutaneous transplantation, compared to BRGS NOD mice , BRGS human mice or can be an effective model for building a more faithful xenotransplant model to study normal and malignant human stem cells
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