Blood: GPS2 promotes red differentiation by regulating EKLF protein stability

Red blood cell production is a complex multi-stage process that involves early erythromycin differentiation to transnuclear mature red blood cells, in which their genealogy-specific transcription factors play an important roleRed blood cell kruppel-like factor (EKLF/KLF1) is a multi-acting red blood cell transcription factor necessary for normal maturation of red blood cells, and its expression and activation are strictly controlled by time and differentiation stage specificityrecently, researchers discovered a new feature in the division of red blood cell cells by G protein signaling pathway inhibitor 2 (GPS2), a subunit of the NCoR/SMRT nuclear receptor auxiliary inhibitor complexthe study found that knocking down GPS2 significantly inhibited in vitro culture and red line differentiation in human CD34-plus cells transplanted into NOD/SCID/IL2Rnull(NSG) miceIn addition, knocking out GPS2 in mice can cause damage to red blood cell production in the liver of the fetal mouse, leading to severe anemiaFlow cell analysis and Wright-Giemsa staining showed defects in late differentiation of Gps2-/-embryonic red blood cell generationmechanism, GPS2 interacts with EKLF to block the degradation of EKLF's protease-mediated, thus increasing the stability and translation activity of EKLFIn addition, the researchers identified the 191-230 amino acid region of the EKLF protein, which is where GPS2 binds to it, which is highly conservative in mammals and is critical to the stability of the EKLF proteinin general, the study revealed a previously unknown role of GPS2 as a post-translation regulator, which enhances the stability of the EKLF protein and promotes red blood cell differentiation