Blood: CLL's high-order immunoglobulin genealogy limitations: stereotyped subsypes s2 #169.

Chronic lymphoblastic leukemia (CLL) is a major stereotyped subtype #2 (IGHV3-21/IGLV3-21, or about 2.5% of all DLLs) and is an aggressive variant of the disease, independent of the soyacyte supermotant (SHM) state of the cloned IGHV gene.
secondary stereotyped subtype #169 (IGHV3-48/IGLV3-21, or about 0.2% of all CLL) is associated with the subtype, which shares a highly similar variable antigen binding point with the subtype.
in this study, Gemenetzi and others explored this relationship in depth through second-generation sequencing and crystallization cloning of B cell-like immunoglobulin (BcR IG).
in the context of the ongoing SHM, evolution through the branch of the main clone type of in-clone diversity (ID) is evident in both subsypes of heavy-chain and light-chain genes.
at the clone or subclonal level, shared SMS was found in heavy- and light-chain genes in all the analyzed cases, highlighting the molecular similarities between the two subtypes.
Particularly noteworthy is the prevalence of an SHM in the link region between the variable and conservative domains of the IGLV3-21 light chain, which has been reported in previous studies as critical to IG-like interactions based on cellular auto-signaling capabilities.
In addition, crystal analysis showed that IG carrying IGLV3-21's CLL subtype 169 retained the same geometry observed in the subtype and the contact residuals involved in the interaction between the same molecules, including SHM in the connecting region;
overall, the study found that stereotyped subtypes s2 and s169 were documented as closely related, with shared IG characteristics that can only be interpreted in the upstream and downstream of the common functional selection.
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