 Home > Biochemistry News > Plant Extracts News > Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4,11-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids

Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4,11-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids

 Last Update: 2021-01-14
 Source: Internet
 Author: User

Tags

host

ps 12 3

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Availability of isotope-labelled metabolites often proves to be an essential prerequisite for the successful identification of a biosynthetic pathway. Also for the metabolic engineering isotope-labelled, either radioactive or non-radioactive, biosynthetic intermediates represent valuable tools for the assessment of metabolic flux, identification of unexpected biosynthetic side reactions, or for the confirmation of the functionality of the engineered reaction step. Most often the required compounds are neither available commercially nor prepared by chemical means within an acceptable time span and effort. Biocatalytic synthesis of early pathway intermediates may offer an attractive solution for this problem. For the metabolic engineering of taxol biosynthesis in a heterologous host, like yeast, isotope-labelled taxanes represent useful tools for the establishment and functional assessment of the introduced biosynthetic steps. Using
Taxus chinensis
taxadiene synthase expressed in the heterologous organism
Pichia pastoris
, we describe a method for the biocatalytic synthesis of tritium (
3
H)-labelled taxa-4(5),11(12)-diene, which represents the pathway committing biosynthetic precursor for all taxoid diterpenoids.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Ginsenoside-Rg5

CAS No.: 186763-78-0

Industrial grade

$1/KG EXW

3-O-Ethyl-L-ascorbic acid /86404-04-8

CAS No.: 86404-04-8

pharmaceutical Grade

$10/KG FOB

TETRAHYDROPIPERINE / 23434-88-0

CAS No.: 23434-88-0

pharmaceutical Grade

$10/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.