Bacteria save common knowledge questions.

1, bacteria preservation tube can save mold and yeast? A: Yes.
For mold and other fungi need to be cultured for 7 days to save, and yeast needs to be cultured for three days to wash off the spores for preservation, the small porcelain beads in the preservation tube can be used as a medium to adsorption spores and mycelium, equivalent to sand preservation method sand as a carrier to play a role.
2, can all bacteria be preserved? If not, what kind of bacteria can be preserved? What kind of bacteria can't be preserved? A: Yes. the principle of
species preservation is to prevent or slow DNA replication when spontaneous mutations lead to the degradation of the strain, so to create a metabolic inactivity state, and the preservation of the bacteria into a dormant state (spores, spores), and create a dry, low temperature, hypoxia, nutrient-deficient environment, the closer the environment created, the longer the preserved species, so in principle, all strains can be saved this way, However, it is important to note that harsh bacteria (e.g. Haemophilus influenzae, meningitis, Ceres) and other special bacteria need to be preserved at a temperature of -80 degrees C or lower, in order to achieve longer-term preservation.
3, can the strain be permanently preserved with a strain preservation tube? A: The lower the temperature, the better the preservation effect.
From the current experimental data and actual preservation effect, -20 degrees C can be stored for 1-5 years, -80 degrees C or liquid nitrogen can be stored for 5-10 years, different strains and different preservation temperatures will vary (e.g., general bacteria such as large intestine, salmon - 20 degrees C we can currently save up to 6 years).
, however, it is important to note that the preserved strains need to be tested annually for the identification of biochemical characteristics to determine that the strains are not mutated.
the separation purification when the bacteria are in 4 and the bacteria are protected? A: It is only after separation and purification that a single strain can be obtained, it is meaningful for a single strain to preserve the strain and prevent cross-contamination and variation of the strain, which is also the basic principle of the preservation of the strain.
5, bacteria preservation tube for the tablet, for the selection of the number of bacteria there are requirements? Or scrape the entire plate of mylocal fallout into the tube? A: For the bacteria to be preserved is generally to be purified 2-3 times, to ensure that a plate of bacteria from a single bacterium, so in the process of bacterial preservation, in order to ensure that the prepared bacteria suspension can reach 3-4MCF , is the entire plate of bacteria can be scraped into the tube, if the plate length is more than half a plate is enough, the specific dosage is also different according to the bacteria, such as mold, yeast in the preservation, not suitable for turbidity method.
6, for liquid culture culture of pure bacteria, can also use our strain preservation tube? If so, how? A: Yes, if the liquid medium can be guaranteed to be pure bacteria, you can directly centrifle, discard the upper clear, and then suck the liquid in the preservation tube into a full mix, and then transfer to the bacteria preservation tube, the whole process should pay attention to sterile operation.
, however, it is best to choose a flat microbiosis for preservation because the bacteria in the liquid culturebone are not easy to observe.
7, why in the preservation of bacteria, to be upside down? Why can't you shake? A: Upside down, can make the bacteria suspension fully mixed, the strain fully and evenly adsorption to the small porcelain beads, spin is not conducive to the bacteria suspension fully mixed, but will make all strains precipitated at the bottom of the tube.
8. Why suck as much liquid out of the tube as possible when using it? A: Sucking out the liquid can avoid the damage of ice crystals formed by repeated freezing and melting to the strain and speed up the recovery process, so it is expected to be absorbed as much as possible after mixing with the bacteria.
commonly used glycerin method, in the process of resuscitation need to use a water bath pot to dissolve quickly, in the process, the strain damage is greater.
9, after use, the preservation environment of the bacteria preservation tube? Do I need a refrigerator at -80C? A: The company's bacteria preservation tube is using low-temperature cryoprotective method, therefore, after use, need to be placed in a low-temperature environment, such as -20 degrees C, -80 degrees C or liquid nitrogen preservation, and the lower the temperature, the better the preservation effect.
users need to select the specific preservation temperature according to the preservation requirements of the bacteria.
10. Why, if available, is it best to place the strain preservation tube in a -80 refrigerator or liquid nitrogen? A: Because storage at low temperatures below -70 degrees C can reduce ice crystal formation, it can greatly reduce the damage caused to bacteria by ice crystal formation, while creating a lower metabolic environment for preserved strains, thus prolonging the time of bacterial preservation.
11, why is the procedure required to be fast when accessing strains? A: The principle of resuscitation of strain preservation requires slow freezing and rapid melting, the reason why it is required to be fast is that in the process of resuscitation of strains, the long dissolution process will cause damage to the preserved strains, and we have improved the preservation method to minimize the impact of resuscitation, but we still recommend to follow the principle of preservation and recovery of bacteria, to ensure the recovery rate of the resuscitating species.
12, the strain will be repeatedly frozen, in the end what impact on the strain? A: Low temperatures cause water in the cells of the strain to form ice crystals, which can cause damage to cellular structures, especially cell membranes.
cells are generally more sensitive to low temperatures than smaller ones, while those without cell walls are more sensitive than those with cell walls.
if placed at low temperature (not a normal refrigerator) for freezing, will produce ice crystals, ice crystals needle-like, easily lead to serious damage to bacteria.
observation with an electric mirror, the visible bacteria's nuclear membrane has a large number of needle-tip-like holes, when removed from low temperatures and began to heat up, ice crystals will become bigger, so repeated freezing, will cause great damage to the strain.
13. Why do bacteria sometimes not save well? A: 1, may be due to the preservation of the liquid did not suck up, repeated freezing and thawing to form ice crystal damage strain cells, affecting the activity of the strain.
2, to determine whether the preserved strain is a number of long-term strains, we recommend that ordinary strains be preserved for 18 hours of fresh strains, and mold needs to wait for spores to appear after the preservation of their spores.
14, can the product be used for the transport of strains, if under what conditions? A: As far as current test data are concerned, we recommend that it be mainly used for short-term transportation of common bacteria, and that anaerobic bacteria should preferably be transported in an anaerobic environment for no more than 5 days at 2-8 degrees C.
it should also be noted that, for example, vibrio genus and erythrosis need to be transported at room temperature, which is extremely sensitive to temperatures of 2-8 degrees C.
.