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    Home > Biochemistry News > Plant Extracts News > Auxin Analysis

    Auxin Analysis

    • Last Update: 2020-11-15
    • Source: Internet
    • Author: User
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    Plant hormones are present and active in minor concentrations in plant tissue. To allow quantification in a restricted amount of material, i.e., protoplasts, chloroplasts, seedlings, seeds, buds, or apical root- and stem regions, a sensitive analytical technique is a prerequisite. During the last two decades, procedures for phytohormone analysis as well as the available hardware have improved substantially. Fluorimetry is widely used for the analysis of indole-3-acetic acid (IAA) because it is more specific and therefore more sensitive than ultraviolet detection (for a review
    seeref.1
    ). Good detection limits for derivatized IAA are obtained in Electron Impact (EI
    +
    ) (
    2
    ,
    3
    ) or negative ion chemical ionization (NICI) GC-MS
    *
    (
    4
    ,
    5
    ).
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