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Plant hormones are present and active in minor concentrations in plant tissue. To allow quantification in a restricted amount of material, i.e., protoplasts, chloroplasts, seedlings, seeds, buds, or apical root- and stem regions, a sensitive analytical technique is a prerequisite. During the last two decades, procedures for phytohormone analysis as well as the available hardware have improved substantially. Fluorimetry is widely used for the analysis of indole-3-acetic acid (IAA) because it is more specific and therefore more sensitive than ultraviolet detection (for a review
seeref.1
). Good detection limits for derivatized IAA are obtained in Electron Impact (EI
+
) (
2
,
3
) or negative ion chemical ionization (NICI) GC-MS
*
(
4
,
5
).