Artemisinin esters

Extraction source: This product is the above-ground extraction of the pyrethronic plant artemisia annua Lester, the total ester content of the intraesterThe raw material is produced in Hunan, Guangxi, Chongqing, Guizhou, and Shaanxi, Hebei and other northern regions have low content.main ingredients arteannuin arteannuin, content: 60%p style"text-align: center; Height: 179px; " .".) Artemisinin Qinghaosu IArtemisinin ethyl Qinghaosu IIArtemisino IArtemisino IArtemisino III, artemisinin IV, artemisinin V, artemisinin G Arteannuin III, Arteannuin IV, Arteannuin V, Arteannuin Gcontent: 20%.the characteristics are white to pale yellow crystalline silk powder, light body light , soluble in ether, chloroform, ethyl acetate, benzene, acetone, ethanol, methanolidentification" take this product and ethanol to make 0.5% solution for test ingeTake the standard artemisinin, artemisinin, artemisinin acetin, artemisinin acetin, artemisinin and other ethanol to make each containing 1mg/ml solution for controlTake artemisinin standard control of 1.0g plus ether 40 ml, ultrasonic extraction for 30 minutes, filter to wave away ether, residue plus ethanol 1 ml dissolved as a control liquidTake the above three liquids each 5 sl, respectively, point on the same silicone G board, to oil ether -ether (4:5), spray 2% vanilla aldehyde 10% sulfuric acid ethanol solution, hot wind, UV lamp 365nm and sunlight inspectionThe corresponding position of the standard speckle appears the same color fluorescent spots and visible spots, compared to the standard medicinal spots, the corresponding rate of 60%Dry weightlessness: No more than 1.0% (Appendix IX.G) burning residue: No more than 0.1% (Appendix IX.J) Heavy Metals: No more than 10ppm (Appendix IX.E) "Content Measurement" according to high-efficiency liquid chromatography (Appendix VI.D) determination chromatography condition C18 column flow phase: 0.01mol/L Na2HPO4-NaH2P04 buffer-methanol (50:50) detection wavelength: 260nm standard curve to take artemisinin standard 100.50mg plus ethanol dissolved at 100ml, take 1.0ml plus 0.02% NaOH4ml, react for 30 min in a water bath at 45C, quickly cool to room temperature, add 0.08/L acetic acid set at 10ml, formulated to 0.1005mg/ml control fluid The injection liquid chromatograph is measured by 1 ?l, 2 ?l, 4 ?l, 6 ?l, 8 ?l, 10 ?l, and 20 ?l injection chromatograph, with concentration as horizontal coordinates, peak area ordinate, and graphed regression equation: Y-3.122496 x (-10000000) x 3.501127 x 100000 linear range: 0.1005 to 0.2010?g sample determination: take this product plus ethanol to make 0.2010mg/ml solution, take 1.0 ml, add 0.02% NaOH 4 ml, react for 30 minutes in a water bath at 45 c, cool the flowing water to room temperature, add 0.08mol/L acetic acid to 10 ml, take 10 l injection liquid chromatography, the standard curve content, and calculate the total ester content the main treatment of the function to enhance immunity, anti-endotoxin For malaria, influenza, schistosomiasis, tumors, parasitic diseases, arrhythmia storage is cool, dry and light-proof