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Applichem Mycoplasma Contamination Solution Gives Cells More Safety and Protection

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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What is Mycoplasma? Why pay attention to mycoplasma contamination in cell culture? A worldwide problem! ! ! ! Literature published by Nature shows serious problem of mycoplasma contamination in cell research field
Ewen Callaway.
Contamination hits cell work.
Nature, 29 July 2014; doi:10.
1038/511518a How can cells be protected from mycoplasma contamination? 1.
AppliChem Mycoplasma Clearance Series

name	main ingredient	Applicable samples	use method
Myco-3 (Cat. No. A5240)	Ciprofloxacin	Adherent cells that clear most mycoplasma contamination	100-fold dilution was added to the medium, the medium was changed every 2-3 days, and the treatment was continued for 14 days
Myco-1 (Cat. No. A5222)	Tiamulin	Adherent cells, together can resolve recalcitrant mycoplasma or mycoplasma contamination after treatment with Myco-3 is discontinued	Add 100-fold dilution to medium for use with Myco-2 . Use Myco-1 for 4 days and then Myco-2 for 3 days, repeat 2-3 times
Myco-2 (Cat. No. A5233)	minocycline

2.
AppliChem Cell Contamination Prevention Series

The best way to effectively protect cells is to prevent the cell culture environment, such as regular disinfection of the working environment, strict experimental operations, and ensuring the sterility of utensils
.
In the laboratory, CO2 incubators, CO2 incubator water trays, and water baths are very important sources of pollution
.
AppliChem has developed a series of pollution prevention products specifically for these 3 major pollution sources, which can effectively prevent fungi (and spores), bacteria, viruses and other pollution
.
Easy to use, non-corrosive, safe and efficient
.

name	Applicable environment	microbe prevention	use method
Incubator-Clean (Cat. No. A5230)	CO2 incubators, work stations, etc.	Mycoplasma, fungi and spores, bacteria, viruses (including HIV, HBV, etc. )	Ready-to-use, spray treatment can be completely wet, no need to transfer cells, it is recommended to treat every 2 weeks
Incuwater-Clean (Cat. No. A5219)	C02 Incubator Water Tray	Mycoplasma, fungi and spores, bacteria, viruses (including HIV, HBV, etc. )	100-fold dilution into the water tray sterile water, it is recommended to replace the sterile water every 2-4 weeks
Aquabator-Clean (Cat. No. A9390)	Water bath	bacteria, fungi	100-fold dilution into the water bath, it is recommended to add it to the water bath every 1-2 weeks, and change the water to the water bath every 4-6 weeks

3.
Applichem Mycoplasma Detection Series

Product number	Detection method	Applicable instruments	Kit Components
PCR Mycoplasma Test Kit (Cat. No. A3744)	Ordinary PCR-electrophoresis detection	General PCR instrument	Reaction Mix Buffer Solution Positive Template Control
qPCR Mycoplasma Test Kit (Cat. No. A9019)	real-time PCR	LightCycler®1. 2, 1. 5, 2. 0, 480, Rotor-Gene™ 3000, 6000, ABI Prism® 7000, iCycler iQ®, iQ™5, Opticon 2, Chromo 4, MX300P®, MX4000®.	Primer/Probe/Nucleotide Mix(RED) Rehydration Buffer(BLUE) Positive Control DNA(GREEN) Internal Control DNA(YELLOW) PCR Grade Water(WHITE)

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