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Make it EvidentSearch for the "switch" of membrane cleavage activity of materials
This issue launches【 SpinSR Makes Cells Evident 】
The author of this article: Dr.
How to realize the precise regulation of the cleavage activity of the material membrane under the slight difference is the key and technical bottleneck of this kind of material in the application of tumor therapy
【Membrane lysis active material】
Membrane lysis active materials can bypass intracellular signaling pathways and induce cell death by targeting the destruction of the cell membrane, effectively overcoming the heterogeneity and drug resistance of tumor cells
However, these materials are also highly toxic to normal tissue cells, and currently they can only be administered by intratumoral injection in clinical practice; the key to their application is to achieve high selectivity for target cells while reducing the toxicity to normal tissue cells.
At present, membrane lysis active materials are mainly used in antibacterial aspects
However, the composition of tumor cell membranes is not significantly different from that of normal tissue cells; in addition, although tumor tissue has a special microenvironment compared to normal tissue, the difference between them is small, such as normal tissue pH is about 7.
How to realize the precise regulation of the cleavage activity of the material membrane under the slight difference is the key and technical bottleneck of this kind of material in the application of tumor therapy
The optimal material P(C6-Bn 20 ) obtained by screening can achieve >32-fold change in cytotoxicity under an input signal of 0.
In this work, the design of pTNT achieves precise control of the function of membrane cleavage active materials at the 0.
Real-time observation with spinning disk confocal SpinSR10
The membrane rupture process of cy5-labeled P(C6-Bn 20 ):
Figure a.
Figure c.
Its image resolution can reach 110 nm; the fast image acquisition of 200 frames per second can quickly capture various changes occurring in living cells;
The real-time controller achieves microsecond-level synchronization of the laser and camera imaging, which greatly reduces photobleaching and phototoxicity, and keeps the cells in a healthy state; the continuous autofocus function automatically tracks the focus position in real time and obtains clear time-lapse images; at the same time Equipped with powerful image analysis and processing software to provide convenience for later data processing;
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