Application of concentration + purification nanofiltration membrane technology in biological fermentation

As a major industrial system of modern biotechnology, the fermentation industry is a large industrial system
that combines traditional fermentation technology and modern NEW technologies such as DNA recombination and cell fusion, and produces useful substances or is directly used for industrial production through modern engineering technology.

At present, the methods of separation and refining of fermentation products in the fermentation industry mainly include precipitation method, salt analysis method, solvent extraction method, adsorption method and so on
.

In addition to these main separation processes, they must also be supplemented by processes such as bacterial isolation, concentration, decolorization, and crystallization
.

In order to improve product quality, reduce costs, increase yield and shorten processing time, the existing post-treatment processes and methods need to be further studied and improved
.

At present, the bio-fermentation concentration and purification process mainly uses nanofiltration membranes
with a molecular weight of 100~1000Dal.

The main feature of the nanofiltration membrane is that the interception performance of divalent ions, functional sugars, small molecular pigments, polypeptides and other substances is higher than 98%, and some monovalent ions, small molecule acids and alkalis, alcohols, etc.
have 30 to 50% permeability performance, and are often used in the classification of solutes, the elution of low molecular substances in the solution and the adjustment of ionic components, the concentration of the solution system and other substances in the separation, refining and concentration process
.

Advantages of concentration and purification by using nanofiltration membrane separation technology: 1.
Low energy consumption and saving the cost of
the concentration process.

2, the process has no chemical reaction, no phase change, does not bring in other impurities and cause decomposition and degeneration of
the product.

3.
Achieve the purpose of concentration and purification at room temperature, without causing damage to the active ingredients, and the yield of the process is high
.

4.
The salt content of the product can be completely removed and the ash of the product can be reduced
.

5, easy to operate, can achieve automatic operation, good stability, easy
maintenance.

Nanofiltration membrane separation technology is often used to replace the traditional process of freeze drying, film evaporation, ion exchange desalination, resin process concentration, neutralization and other processes
.

At the same time, it can remove suspended particles, bacteria, tannin, starch, colloids, proteins, some pigments and other macromolecules, with high clarity and improve product purity
.