Application of Centrifuge in Microbiological Diagnosis Separation and Cultivation Technology

In microbiological diagnosis, centrifuges are often used to separate the tested microorganisms from the tested materials and obtain pure culture (ie, a single microorganism culture)
.

Therefore, centrifuges are generally used to centrifuge samples.
The centrifuge separation and culture of microorganisms is a key operation method in microbiological diagnosis
.

There are many ways to isolate a single microorganism, and the common ones are as follows: 1.
Inoculate the tested material into a suitable microbial culture medium, and then select a single microbial cell from the bacteria to be isolated from the bacteria generated on the surface of the solid culture medium.
Transplant into another suitable microbial medium for cultivation
.

Since one bacterial cell is generally produced by the reproduction of one bacteria, the cultured bacteria are of a single type, that is, pure culture
.

2.
Use a unique culture environment (such as anaerobic, carbon dioxide environment, etc.
) to separate bacteria
.

The inoculum of the material to be inspected is susceptible to the body of the animal, so that the pathogenic bacteria can multiply in the body of the animal, and then take the material from the body of the animal and use a centrifuge to separate the required components for cultivation
.

Using some bacteria to have unique resistance to some physical and chemical elements, dispose of the inoculum by physical and chemical methods, kill other microorganisms and retain the required bacteria, and then separate and culture
.

 　　Usually, the tested materials can be separated and cultured by the first two methods
.

If the pollution of the collected materials is serious, the latter two methods can be used to dispose of them first, and then the first two methods can be used to obtain pure culture
.

 　　In the isolation and culture operation, strict aseptic operation is performed
.

All supplies, microbial culture media, and reagents must be sterilized and cannot be exposed to air for a long time
.

It is strictly forbidden to touch or blow the inside of the sterilized utensils with your hands
.

 　　According to the characteristics of the pathogenic seedlings that may exist in the tested materials, select the appropriate microbial medium and culture conditions: (temperature, gas environment, etc.
)
.

For the initial isolation of unknown bacteria, it is best to use several microbial media, such as broth, blood agar, McConk agar, etc.
, inoculate several tubes of each microbial culture medium, and place them in a normal environment and an anaerobic environment.
cultivated
in.

Usually, the results are observed within 24-78 hours, but there are still some pathogenic bacteria, which can only be generated after a long time of culture
.

 　　The tested materials usually do not need to be disposed of, and can be directly planted on the microbial culture medium
.

When it is suspected to be a pathogenic seedling with budding brain, the tested material can be ground with normal saline to make a 1:10 emulsion (liquid material does not need to be diluted), and placed in a 60-80 ℃ water bath for 20-30 minutes to kill Bacteria without spores are then inoculated into the microbial medium
.