Application case of SPE solid phase extraction unit

SPE is one of the effective purification methods when the sample is very complex It is mainly used for pretreatment of samples, extraction of semi-volatile or non-volatile compounds, or removal of impurities in samples that cause interference to separation and analysis, and also for treatment of solid samples that can be pre dissolved into solvents Compared with the traditional liquid-liquid extraction method, it can improve the recovery rate of the analyte, and more effectively separate the analyte and the interference component, shorten the sample pretreatment time and make the operation simple The application field can be applied to all kinds of food safety testing, agricultural product residue monitoring, medicine and health, environmental protection, commodity inspection, tap water and chemical production laboratory Bisphenol A, also known as BPA, is used to synthesize polycarbonate (PC) and epoxy resin in industry Since the 1960s, it has been used to make plastic (milk) bottles, suction cups for children, and inner coating of food and beverage (milk powder) cans BPA is everywhere, from mineral water bottles, medical devices to food packaging But BPA can also lead to endocrine disorders, threatening the health of the fetus and children Obesity caused by metabolic disorders is also thought to be related The European Union believes that the baby bottles containing BPA will induce precocious puberty From March 2, 2011, the baby bottles containing BPA are prohibited In the pre-treatment process of bisphenol a detection in bisphenol a molecular structure plastic products, take 5g of plastic sample, cut it into 5 × 5mm small pieces, place it in a 250ml triangle bottle, add 100ml of methanol, extract it by ultrasonic at 50 ℃ for 2h, filter it into 250ml chicken heart bottle, rotate and evaporate it to dryness, elute with 5ml of water vortex, wait for purification Sample concentration and purification SPE column: C18 500mg / 3ml activation, balance: 10ml methanol, 10ml water successively activate the sample: when the water reaches the upper sieve plate of the small column, add the liquid to be purified Rinsing: 6ml of water and 10ml of 30% methanol aqueous solution are used to rinse the column in turn, and the effluent is discarded Elution: 6 ml of 50% acetonitrile aqueous solution was eluted and the eluate was collected The eluate was blown to near dry by nitrogen blower at 50 ℃, dissolved by 1ml mobile phase, and analyzed by HPLC.