Application and operation method of Escherichia coli tube centrifuge

The important steps of extracting biochemical substances from the fermentation broth or cell culture broth of Escherichia coli genetically engineered bacteria are pretreatment and solid-liquid separation
.

The purpose is not only to separate the genetically engineered bacteria of E.
coli and other suspended particles, but also to remove some soluble impurities and change the properties of the fermentation broth, so as to facilitate the subsequent extraction of metabolites from the clarified fermentation broth by physical and chemical methods, or Cell disruption, separation of fragments and extraction of intracellular products are carried out from Escherichia coli genetically engineered bacteria
.

There are many impurities in the fermentation broth of Escherichia coli genetically engineered bacteria, some of which not only directly affect the product quality and yield, but also have a great impact on the subsequent extraction and refining.
Therefore, during pretreatment, appropriate methods must be used to precipitate these impurities.
, which is removed during solid-liquid separation, so as to facilitate the smooth progress of subsequent extraction and refining processes
.

Centrifugal separation is mainly used for the solid-liquid separation of the fermentation broth of E.
coli genetically engineered bacteria.
We use the centrifugal force generated by the high-speed rotation of the E.
coli tube centrifuge to achieve the solid-liquid separation of the fermentation broth.
Good phase clarity and other advantages
.

Operation method of Escherichia coli tube centrifuge: When the Escherichia coli tube centrifuge is operated, the material to be processed enters the bottom of the drum from the feed pipe through the bottom hollow shaft under a certain pressure (about 3 × 104Pa), and is folded by a circular shape.
Turning baffles are scattered around the drum
.

In order to keep the liquid from leaving the drum wall, a cross-shaped baffle is arranged in the drum, and the liquid is accelerated to the drum speed by the baffle in the drum.
Under the centrifugal force field, the turbid liquid (or suspension) moves along the shaft.
In the process of upward flow, it is stratified into a light liquid phase and a heavy liquid phase (or liquid phase and solid phase)
.

And it is discharged through the upper annular overflow port
.

Changing the inner diameter of the separation ring at the upper end of the drum can adjust the layered interface of the heavy liquid phase and the light liquid phase
.

When handling the suspension, the heavy liquid port of the tubular centrifuge can be closed, leaving only the central light liquid overflow port, and the solids will be deposited on the dry drum wall under the centrifugal force field
.