Apoptosis induced by silybin (sb)

2012-11-07 classification: efficacy 0 people comment on apoptosis and its related molecular events, which play an important role in tumor occurrence, development and treatment Inducing apoptosis has become an effective method of anticancer treatment Tyagi AK et al Observed that RT4 cells treated with silybin (sb) could induce apoptosis The apoptosis rate of silybin (sb) (100 μ mol / ml) for 24 hours was 41%, while that of the control group was only 6%, accompanied by the activation of caspase-9 and Caspase-3 and the cleavage of ADP ribose polymerase (PARP) Further detection showed that the level of survivin protein, as an inhibitor of apoptosis, was significantly reduced, while the concentration of silybin (sb) at 200 μ mol / ml for 24 and 48 hours, survivin could be completely disappeared RT-PCR showed that the mRNA level of survivin protein decreased year on year Tyagia et al Found that silybin (sb) could induce apoptosis of tcc-sup cells in bladder cancer, which was time-dependent and dose-dependent Caspase-3 and PARP were detected Mohan s et al Observed the protective effect of silibinin (sb) on skin cancer induced by ultraviolet rays It was found that local application of silibinin (sb) before irradiation could increase the apoptosis induced by UV B, the activation of caspase-9, caspase-3 and capase-7, the release of cytochrome c, and the up regulation of Bax, Bak and the down regulation of bcl-2 In addition, silibinin (sb) can induce apoptosis of colon cancer HT-29 cells, but no activation of Caspase-3, caspase-9 and PARP and release of cytochrome C were observed Z-val-ala-asp-ome-fmk, the full inhibitor of caspase, failed to reverse the apoptosis induced by silibinin (sb), indicating that it is non caspase dependent Mitogen activated protein kinase (MAPK) is an important molecule in cell signal transduction, which is widely involved in cell differentiation, proliferation, malignant transformation and apoptosis Extracellular regulatory protein kinase (ERK) and c-jun amino terminal kinase in MAPK are closely related to cell apoptosis Singh RP et al Fed SENCAR mice with dermal papillomas with diets supplemented with silybin (sb) and silymarin (SM), found that they could significantly inhibit tumor growth and apoptosis of a large number of tumor cells The results showed that the activity of erki / 2 and jnki / 2 decreased significantly, but did not change their protein level; p38MAPK was slightly activated, suggesting that the apoptosis was related to the change of signal transduction level TNF-a can induce tumor cell apoptosis and activate the expression of NF XB gene related to cell survival, while NF-kB can prevent TNF mediated apoptosis Dhanalakshmi s et al Observed that silybin (sb) can inhibit TNF induced NF-kB activation and promote tnf-a-induced apoptosis in DU 145 cells Analysis showed that silybin (sb) significantly enhanced the expression of NF-kB inhibitor protein (IKBS), inhibited the degradation of IKBS and increased its content in cells Silibinin (sb) also interfered with the translocation of NF KB subunits p65 and p50 from cytoplasm to nucleus, which reduced the level of p65 and P50 in nucleus and affected the gene activation.