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Analyzing Plant Signaling Phospholipids Through 32Pi-Labeling and TLC

 Last Update: 2020-11-13
 Source: Internet
 Author: User

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Lipidomic analyses through LC-, GC-, and ESI-MS/MS can detect numerous lipid species based on headgroup and fatty acid compositions but usually miss the minor phospholipids involved in cell signaling because of their low chemical abundancy. Due to their high turnover, these signaling lipids are, however, readily picked up by labeling plant material with
32
P-orthophosphate and subsequent analysis of the lipid extracts by thin layer chromatography. Here, protocols are described for suspension-cultured tobacco BY-2 cells, young
Arabidopsis
seedlings,
Vicia faba
roots, and
Arabidopsis
leaf disks, which can easily be modified for other plant species and tissues.

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