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Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics

 Last Update: 2020-11-19
 Source: Internet
 Author: User

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In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based on
SDS
-PAGE fractionation of proteins. Rice proteomics has benefitted substantially from successful execution of shotgun proteomics techniques. We describe steps on how to proceed starting from rice protein extraction, SDS-PAGE, in-gel protein digestion with trypsin, nanoLC-MS/MS, and database searching using the GPM. Data from these experiments can be used for spectral counting, where simultaneous quantitation of several thousand proteins can be obtained.

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