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Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing

 Last Update: 2021-03-10
 Source: Internet
 Author: User

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what norwalk virus

gene expression profiling

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Virus-induced gene silencing (VIGS) is a powerful RNA-silencing based technology adapted for the study of host-gene function. VIGS functions through the expression of a host gene from a virus vector. Both the virus-encoded host sequence and the homologous host target messenger RNA are destroyed or made inactive through a host surveillance system. Here, we describe procedures for the use of a new virus vector for VIGS in monocotyledonous hosts and, in particular, in rice (
Oryza sativa
), a species for which no VIGS vector was previously available.

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