Anaerobic incubator instructions

The anaerobic environment of the operation room forms 1) The necessary accessories and appliances are placed according to the requirements of use, and two non-toxic plastic bags
are placed into the operation room.

2) Mixed gas cylinder, nitrogen cylinder input pressure adjustment, adjust the pressure reducing valve, so that the input pressure is 0.
1Mpa3) after opening the equipment, after the power switch, press the total power button on the control board to make the equipment energized
.

4) Put 1000g palladium granules (sealed) into the operation chamber, dehumidify by the condensation system, and put in the anaerobic indicator
.

5) Close the indoor and outdoor doors of the sampling and vacuum calibration
.

(Press the vacuum pump key on the control face card, press on, press off) 6) The first displacement (nitrogen displacement) in the operating room :(1) first insert the rubber tube into the air intake in the operating room, and the other into the plastic bag
.

(2) Connect the nitrogen intake path, open the nitrogen control valve in the operating room, make the two plastic bags full of nitrogen, close the solenoid valve, and then tighten the bag mouth
.

(3) Put the latex gloves on the flange ring of the observation plate and tie it tightly
.

(4) The nitrogen in the plastic bag is gradually placed in the operating room until all are removed
.

7) The second displacement (nitrogen displacement) of the operation chamber repeats the nitrogen flushing process, the sampling chamber is first evacuated, and pay attention to closing the exhaust at any time by pressing the exhaust valve
.

8) The third replacement of the operation chamber (mixed gas displacement) (the mixture gas ratio is H2 1/85% H2 1/10% H2 1/10% CO2/15%) (1) The transfer and exchange path opens the intake of the mixing valve in the operating room, and the sampling chamber is first evacuated when flushing, and pay attention to closing the exhaust at any time according to the exhaust valve
.

(2) After the mixture is filled with plastic bags, close the solenoid valve and open the mixture flow limiting valve, so that the mixture passes through the flow stabilizer, flow meter, and adjustment flow meter, and the flow rate is about
10 ml per minute.

(3) Gradually discharge the mixture in the plastic bag in the operating room
.

(4) After three air changes, the oxygen content of the gas in the operating room has been in an extreme state 9) open the palladium particle deaerator in the operating room, turn on the power supply of the deaeration catalyst for catalytic deoxygenation, and drop a little clean water on the reaction area of the anaerobic indicator strip, after 1-2 hours, observe its discoloration, and the dark blue part of the reaction becomes light blue to achieve an anaerobic environment
in the operating room.

10) Ultraviolet sterilization lamp, indoor sterilization treatment, sterilization time self-determined
.

Placement and culture of strains 1) Check the door of the sampling chamber and close it
.

2) Open the door outside the sampling room, and close the bacteria after placing them in the sampling room
.

3) The sampling chamber nitrogen filling displacement three times, first pump vacuum 500 mm Hgg (66Kpa) above the stop, and then open the sampling chamber oxygen valve intake, so that the pointer back to zero, close the valve
.

The sampling chamber is filled with nitrogen displacement three times The process ends
.

4) If the selected vacuum is low, the number
of displacements needs to be increased.

5) After the sampling outdoor door is opened and closed, the low vacuum degree of 100 mm Hg (13Kpa) is tested
.

6) The anaerobic incubator requires long-term continuous use of the conditions (1) put the Meilan indicator bar in the operating room every day for observation, and re-ventilate
if it cannot be normal.

(2) It is necessary to continuously input trace amounts of mixed gas
for a long time.

The combined nitrogen energy and trace amounts of nitrogen through catalytic absorption ensures the indoor anaerobic state, and the mixed gas flow rate is selected as about
10 ml per minute.

(3).
Continuous incubation and operation for one day, replace the oxygen remover and desiccant
once.

(The replaced oxygen remover and desiccant can be placed in a 200 °C drying chamber, and drying for 2-3 hours can restore it) 7) Application of inoculation stick sterilizer: (1) Short-circuit the electrode and become red hot at any time with a nickel-soldering electric wire for sterilization (inoculation stick).


(2) The molten wax can be directly placed on the test tube sealing port to rotate the molten wax
.

8) The temperature in the incubator of the incubator of the operation chamber can be arbitrarily selected and controlled
.

Note 1.
The instrument is installed as far as possible in a place where
the air is quiet and the temperature change is small.

2.
Before starting, you should be fully familiar with and understand the instructions of each component of the supporting instruments and meters, and master the correct use method
.

3.
The culture is placed after reaching an anaerobic environment in the operating room
.

4.
If there is a fault (air outage, etc.
), the operating room can still maintain an anaerobic state
for 12 hours.

(After 12 hours, remove the culture for additional treatment as needed).


5.
Always pay attention to whether there is air leakage in
the air path.

6.
When changing the gas cylinder, pay attention to tightening the trachea to avoid flowing into the oxygen-containing body 7.
The vacuum pump is used according to the requirements and regularly checks and refuels
.

8.
Stop using, turn off the total power key, and the power switch on the rear of the device