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Tris is an organic substance with the chemical formula C4H11NO3, white crystalline particles
.
Can be used as biological buffer; used to prepare buffers for gel electrophoresis
.
As an alkaline drug, it is used for the correction of acidosis without causing an increase in carbon dioxide retention
.
Each time 7.
28% THAM 2-3ml/kg (isotonic solution is 3.
64%), diluted with an equal amount of 5%-10% glucose solution and slowly dripped
.
The following editor will briefly introduce to you how to use Tris to make buffer? As shown below: Buffer formula: (0.
5 M pH7.
6) Tris (Tris(Tris(hydroxymethyl)aminomethane)) 60.
57g 1N HCl about 420ml double-distilled water to 1000ml Preparation method Dissolve with a small amount of double-distilled water (300~500ml) first Tris, after adding HCl, adjust the pH to 7.
6 with HCl (1N) or NaOH (1N), and finally add double distilled water to 1000 ml
.
This solution is a stock solution and is stored in a refrigerator at 4°C
.
Note: The pH value of Tris-HCl varies with temperature, so it should be measured at room temperature, so that the measured results are more reliable
.
Uses Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses
.
Tris was used for protein crystal growth at different pH conditions
.
[3] The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C.
elegans
.
[4] Tris is also one of the main components of protein electrophoresis buffers
.
In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators and some pharmaceuticals
.
Tris is also used as a titration standard
.
Buffering properties Tris is a weak base with a pKa of 8.
1 at room temperature (25°C); according to buffer theory, the effective buffering range of Tris buffer is between pH 7.
0 and 9.
2
.
The pH of the aqueous solution of Tris base is about 10.
5.
Generally, hydrochloric acid is added to adjust the pH value to the desired value, and then the buffer solution with the pH value can be obtained
.
However, attention should be paid to the effect of temperature on the pKa of Tris
.
.
Can be used as biological buffer; used to prepare buffers for gel electrophoresis
.
As an alkaline drug, it is used for the correction of acidosis without causing an increase in carbon dioxide retention
.
Each time 7.
28% THAM 2-3ml/kg (isotonic solution is 3.
64%), diluted with an equal amount of 5%-10% glucose solution and slowly dripped
.
The following editor will briefly introduce to you how to use Tris to make buffer? As shown below: Buffer formula: (0.
5 M pH7.
6) Tris (Tris(Tris(hydroxymethyl)aminomethane)) 60.
57g 1N HCl about 420ml double-distilled water to 1000ml Preparation method Dissolve with a small amount of double-distilled water (300~500ml) first Tris, after adding HCl, adjust the pH to 7.
6 with HCl (1N) or NaOH (1N), and finally add double distilled water to 1000 ml
.
This solution is a stock solution and is stored in a refrigerator at 4°C
.
Note: The pH value of Tris-HCl varies with temperature, so it should be measured at room temperature, so that the measured results are more reliable
.
Uses Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses
.
Tris was used for protein crystal growth at different pH conditions
.
[3] The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C.
elegans
.
[4] Tris is also one of the main components of protein electrophoresis buffers
.
In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators and some pharmaceuticals
.
Tris is also used as a titration standard
.
Buffering properties Tris is a weak base with a pKa of 8.
1 at room temperature (25°C); according to buffer theory, the effective buffering range of Tris buffer is between pH 7.
0 and 9.
2
.
The pH of the aqueous solution of Tris base is about 10.
5.
Generally, hydrochloric acid is added to adjust the pH value to the desired value, and then the buffer solution with the pH value can be obtained
.
However, attention should be paid to the effect of temperature on the pKa of Tris
.
