An artemisinin alkaloid on the snow

Extracted Source: This product is aconitum brachypodum Diels (A.flavum Hand.-Mazz.) The total alkaloid content of the alkaloids extracted from the root of the block is 80%

"Main Ingredients" sub-utamine Hypaconine, content: 50%

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" on the snow a branch of artemisin-based bullatine A

neolin Neoline

quasi-guru head base Songorine

glysitrol-alkali Denudine

content: 30%

" this product is white to light yellow powder, soluble in chloroform, benzene, ethanol, meldonium, meldonium, propylene

"Identification" take this product chlorine imitation into 0.5% solution for test solutionTake the standard products of utoamine, snow a branch of artemisinin, light glycenoids and other chlorine imitation into a 1 mg / ml solution as a control solutionTake a branch of artemisinin standard control herbs 1.0g plus ammonia water 1 ml, moist 40 minutes, add chloroform 30 ml, ultrasonic extraction 30 minutes, splending dry, residue heating water 20 ml, boiling for 30 minutes, cooled, chloroform extraction 3 times, combined chloroform extract concentrated to 1 ml of control liquidTake the above three liquids each 5 sl, respectively, point in the same silicone G board, point three boards at a time

(1) to chloroform-methanol-water (15:5:2) underthe expansion, spray improved potassium iodide test fluid

(2) to positive hexane-ethyl acetate-methanol (6.4:3.6:1) to expand, spray thin iodide potassium test fluid

(3) unfolded with petroleum ether-ether (1:10), iodized vapor color rendering

above three plates, the corresponding location of the label spots show the same color spots, compared to the standard medicinal spots, the corresponding rate of 50%

" Check" biestal alkaloids (based on high-efficiency liquid chromatography) assays

chromatographic condition C18 bar

flow phase: A-acetylene-tetrahydrofuran (25:15

B-0.1mol/L ammonium acetate (0.1mD per 1000ml ice acetate)

detection wavelength: 235nm

the number of theoretical boards is 3000

control fluid: the new utobase, the sub-utobase, the utobase plus isopropyl alcohol-dichloromethane (1:1) are made each containing 5 mg/ml solution spare

test solution: Take this product with isopropanol-dichloromethane (1:1) to make a 5 mg/ml solution backup

determination: take the above-mentioned two liquid injection liquid chromatography, the total amount of bieste alkaloids should not exceed 0.002%

dryweight: not more than 3.0% (Appendix IX G)

burning residue: not more than 5.0% (Appendix IX J)

heavy metals: not more than 10 ppm (Appendix IX E)

thin layer conditionth sheet: silicone G plate

expanse: petroleum ether-ether-acetone (5:2:3), ammonia saturated

color rendering: UV light viewing

scan condition wavelength: s s 270nm

r-360nm

scan: serrated scan

scattering parameters: SX s1

standard curve: the sub-uttomine label and chlorine is copied into a 3.3 mg/ml solution Divide 2 sl, 3 sl, 4 sl, 5 sl, 6 sl, point on the same thin plate, expand, UV lamp positioning, scan The horizontal coordinates are as much as the dot sample, and the speckle integral value is the ordinate, which is charted

sample determination: take this product with chlorine imitation into 6.6 mg /ml solution Divide 3 sl, 4 sl and control liquid: 2 sl, 3 sl, 4 sl, 5 sl, cross point on the same thin layer plate, expand, scan The content is calculated on a standard curve and the total alkaloid content is calculated

"functional treatment" analgesia, bureau hemp, anti-inflammatory, anti-tumor, strong heart For rheumatism pain

"storage" cool, dry, to avoid light